Effect of glucose on Treponema denticola cell behavior

Introduction:  Treponema denticola inhabits the oral subgingival environment and is part of a proteolytic benzoyl‐dl‐arginine‐naphthylamide‐positive ‘red complex’ associated with active periodontal disease. Spirochetes have a unique form of chemotactic motility that may contribute to their virulence...

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Bibliographic Details
Published in:Oral microbiology and immunology 2008-06, Vol.23 (3), p.234-238
Main Authors: Ruby, J. D., Lux, R., Shi, W., Charon, N. W., Dasanayake, A.
Format: Article
Language:English
Subjects:
Bacteriological Techniques
Bacteriology
Biological and medical sciences
Blotting, Western
Chemotaxis - drug effects
Culture Media
Dentistry
Electrophoresis, Polyacrylamide Gel
Flagella - chemistry
Flagella - drug effects
Flagellin - analysis
Flagellin - drug effects
Fundamental and applied biological sciences. Psychology
Glucose - pharmacology
glucose chemotaxis
Glycoproteins - analysis
Humans
Microbiology
Microscopy, Video
Miscellaneous
motility
Mouth - microbiology
Mutation - genetics
oral spirochetes
periodontal diseases
Treponema denticola
Treponema denticola - drug effects
Treponema denticola - genetics
Treponema denticola - growth & development
Viscosity
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Summary:Introduction:  Treponema denticola inhabits the oral subgingival environment and is part of a proteolytic benzoyl‐dl‐arginine‐naphthylamide‐positive ‘red complex’ associated with active periodontal disease. Spirochetes have a unique form of chemotactic motility that may contribute to their virulence. Chemotaxis is essential for efficient nutrient‐directed translocation. Methods:  We examined the effect of glucose on T. denticola cell velocity, expression of periplasmic flagella proteins, and chemotaxis, e.g. translocation into capillary tubes. Results:  The presence of glucose did not significantly effect T. denticola cell velocity in high viscosity conditions nor did it alter periplasmic flagella protein expression. The addition of glucose to capillary tubes resulted in greater numbers of T. denticola cells in tubes containing glucose. A non‐motile mutant did not migrate into capillary tubes containing glucose. Conclusion:  These results are consistent with a chemotactic response to glucose that is motility dependent.
ISSN:0902-0055
1399-302X
DOI:10.1111/j.1399-302X.2007.00417.x