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Wnt3a binds to several sFRPs in the nanomolar range

Secreted Frizzled-related proteins (sFRPs) are modulators of the Wnt signaling pathway that plays important roles in both embryogenesis and oncogenesis. sFRPs have been proposed to antagonize Wnt activity by binding to Wnts. However, the affinity of this binding is unknown. Here we show, using surfa...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2007-06, Vol.357 (4), p.1119-1123
Main Authors: Wawrzak, Danuta, Métioui, Mourad, Willems, Erik, Hendrickx, Marijke, Genst, Erwin de, Leyns, Luc
Format: Article
Language:English
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Summary:Secreted Frizzled-related proteins (sFRPs) are modulators of the Wnt signaling pathway that plays important roles in both embryogenesis and oncogenesis. sFRPs have been proposed to antagonize Wnt activity by binding to Wnts. However, the affinity of this binding is unknown. Here we show, using surface plasmon resonance and purified proteins, that sFRP1, sFRP2, sFRP4, and Frzb bind directly to Wnt3a with affinities in the nanomolar range. However, only sFRP1 and sFRP2 antagonize Wnt3a activity by blocking Wnt3a induced β-catenin accumulation in L cells. Furthermore, sFRP2, but not Frzb, antagonizes Wnt3a signaling in an ES cell model of mesoderm differentiation. These results provide the first measurement of binding affinity of sFRPs for a Wnt, which together with the measurement of antagonistic activity of sFRPs could help understand how sFRPs regulate Wnt signaling.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2007.04.069