Homologous chromosome pairing is completed in crossover defective atzip4 mutant

In transposon-tagged lines of Arabidopsis, we found a mutant that was defective in meiotic chromosome segregation. This mutant, named atzip4-4, was due to a novel mutant allele of AtZIP4, which has sequence similarity to yeast ZIP4/SPO22, which codes a ZMM protein that is a proposed unit of the syna...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2008-05, Vol.370 (1), p.98-103
Main Authors: Kuromori, Takashi, Azumi, Yoshitaka, Hayakawa, Shun, Kamiya, Asako, Imura, Yuko, Wada, Takuji, Shinozaki, Kazuo
Format: Article
Language:English
Subjects:
Arabidopsis
Arabidopsis - cytology
Arabidopsis - genetics
Arabidopsis Proteins - analysis
Arabidopsis Proteins - genetics
Arabidopsis Proteins - physiology
AtZIP4
Cation Transport Proteins - analysis
Cation Transport Proteins - genetics
Cation Transport Proteins - physiology
Chiasmata
Chromosome Pairing - genetics
Chromosomes, Plant - chemistry
Chromosomes, Plant - genetics
Crossing Over, Genetic - genetics
Crossover
In Situ Hybridization, Fluorescence
Transposon-tagged line
ZIP4/SPO22
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Summary:In transposon-tagged lines of Arabidopsis, we found a mutant that was defective in meiotic chromosome segregation. This mutant, named atzip4-4, was due to a novel mutant allele of AtZIP4, which has sequence similarity to yeast ZIP4/SPO22, which codes a ZMM protein that is a proposed unit of the synapsis initiation complex. The chiasma distribution in atzip4-4 differed from that in the wild-type, involved in a deficiency of interfering crossovers in the mutant genome. On the other hand, FISH staining of loci on two independent chromosomes in mutant meiocytes indicated that homologous chromosome pairing to synapse progresses normally until the pachytene stage, yet homologous chromosomes often separated abruptly at diplotene and diakinesis. These results suggest that AtZIP4 plays an important role in normal crossover formation and meiotic chromosome segregation, but not in homolog search. The relationship of AtZIP4 and other related proteins in meiotic events is discussed and compared with that in yeast.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2008.03.036