Antitubercular triterpenes and phytosterols from Pandanus tectorius Soland. var. laevis

• Published in: Journal of natural medicines 2008-04, Vol.62 (2), p.232-235
• Main Authors: Tan, Mario A., Takayama, Hiromitsu, Aimi, Norio, Kitajima, Mariko, Franzblau, Scott G., Nonato, Maribel G.
• Format: Article
• Language: English
• Subjects: Antitubercular Agents - chemistry; Antitubercular Agents - isolation & purification; Antitubercular Agents - pharmacology; Biomedical and Life Sciences; Biomedicine; Complementary & Alternative Medicine; Medicinal Chemistry; Microbial Sensitivity Tests; Mycobacterium tuberculosis - drug effects; Mycobacterium tuberculosis - growth & development; Pandanaceae - chemistry; Pharmacology/Toxicology; Pharmacy; Phytosterols - chemistry; Phytosterols - isolation & purification; Phytosterols - pharmacology; Plant Extracts - chemistry; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; Plant Leaves - chemistry; Plant Sciences; Sitosterols - chemistry; Sitosterols - isolation & purification; Sitosterols - pharmacology; Squalene - chemistry; Squalene - isolation & purification; Squalene - pharmacology; Stigmasterol - chemistry; Stigmasterol - isolation & purification; Stigmasterol - pharmacology; Triterpenes - chemistry; Triterpenes - isolation & purification; Triterpenes - pharmacology
• ISSN: 1340-3443; 1861-0293
• DOI: 10.1007/s11418-007-0218-8

Bioassay-guided chromatographic purification of the antitubercular chloroform extract of Pandanus tectorius Soland. var. laevis leaves afforded a new tirucallane-type triterpene, 24,24-dimethyl-5β-tirucall-9(11),25-dien-3-one ( 1 ), squalene and a mixture of the phytosterols stigmasterol and β-sitosterol. Microplate Alamar Blue Assay (MABA) showed that 1 inhibited the growth of Mycobacterium tuberculosis H 37 Rv with a MIC of 64 μg/mL, while squalene and the sterol mixture have MICs of 100 and 128 μg/mL, respectively.