Effect of dietary control on plasma nitrate level and estimation of basal systemic nitric oxide production rate in humans

It is of great interest and value to evaluate the systemic nitric oxide (NO) production rate in humans under various conditions. However, the currently available estimation methods are troublesome and time-consuming. We thus aimed at developing a simple method to estimate the basal systemic NO produ...

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Bibliographic Details
Published in:Heart and vessels 2000-01, Vol.15 (6), p.274-279
Main Authors: Mochizuki, S, Toyota, E, Hiramatsu, O, Kajita, T, Shigeto, F, Takemoto, M, Tanaka, Y, Kawahara, K, Kajiya, F
Format: Article
Language:English
Subjects:
Adult
Body Weight
Diet
Female
Humans
Male
Nitrates - blood
Nitrates - urine
Nitric Oxide - biosynthesis
Nitric Oxide Synthase - metabolism
Time Factors
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Summary:It is of great interest and value to evaluate the systemic nitric oxide (NO) production rate in humans under various conditions. However, the currently available estimation methods are troublesome and time-consuming. We thus aimed at developing a simple method to estimate the basal systemic NO production rate in humans based on a steady-state analysis, i.e., a balance between the systemic NO production rate and the total nitrate elimination rate. Plasma nitrate concentrations of young healthy volunteers (n = 7 in group 1: n = 9 in group 2) were measured for 2 days. In group 1, all subjects had the same meals for 7 days prior to the plasma nitrate measurement. In group 2, all subjects were allowed free diets. The plasma nitrate concentrations were highly influenced by dietary nitrite/nitrate intake in both groups and reached the steady-state levels after 14-h fasting. Accordingly, the basal systemic NO production rates were estimated from the plasma nitrate concentrations after 14-h fasting (group 1, 630 +/- 37 nmol min(-1) = 0.78 +/- 0.03 micromol kg(-1) h(-1); group 2, 597 +/- 45 nmol min(-1) = 0.66 +/- 0.05 micromol kg(-1) h(-1), P = not significant vs group 1). These estimated values were comparable to the values obtained by other methods. In conclusion, the present estimation method with 14-h fasting using a single-compartment analysis was found to be a simple approach to quantitative evaluation and intra- and interindividual comparisons of the basal systemic NO production rates in humans.
ISSN:0910-8327
1615-2573
DOI:10.1007/s003800070005