Pre vivo, ex vivo and in vivo evaluations of [68 Ga]-EDTMP

Abstract Introduction The objectives of this study were to develop a simple preparation method for [68 Ga]-EDTMP and to evaluate the applicability of [68 Ga]-EDTMP as a potential positron emission tomography (PET) bone imaging agent using pre vivo, ex vivo and in vivo models. Methods [68 Ga]-EDTMP w...

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Published in:Nuclear medicine and biology 2007-05, Vol.34 (4), p.391-397
Main Authors: Mitterhauser, Markus, Toegel, Stefan, Wadsak, Wolfgang, Lanzenberger, Rupert R, Mien, Leonhard-Key, Kuntner, Claudia, Wanek, Thomas, Eidherr, Harald, Ettlinger, Dagmar E, Viernstein, Helmut, Kluger, Rainer, Dudczak, Robert, Kletter, Kurt
Format: Article
Language:English
Subjects:
Animals
Bone
Bone and Bones - diagnostic imaging
Bone scan
Fluorine Radioisotopes - pharmacokinetics
Fluorine-18
Gallium Radioisotopes - chemistry
Gallium-68
Humans
Image Processing, Computer-Assisted
Indicators and Reagents
Mice
Organophosphorus Compounds - pharmacokinetics
PET
Positron-Emission Tomography
Radiology
Radiopharmaceuticals - pharmacokinetics
Tissue Distribution
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Summary:Abstract Introduction The objectives of this study were to develop a simple preparation method for [68 Ga]-EDTMP and to evaluate the applicability of [68 Ga]-EDTMP as a potential positron emission tomography (PET) bone imaging agent using pre vivo, ex vivo and in vivo models. Methods [68 Ga]-EDTMP was prepared using [68 Ga]-gallium chloride eluted from the68 Ge/68 Ga generator and commercially available Multibone kits. Binding affinity to bone compartments was evaluated using a recently established pre vivo model. In vivo (microPET) and ex vivo experiments were performed in mice, and the results of which were compared with those obtained with [18 F]-fluoride. Results [68 Ga]-EDTMP was accessible via simple kit preparation and predominantly accumulated in bone tissue in vivo, ex vivo and pre vivo. Binding to mineral bone was irreversible, and low binding was observed in organic bone. In vivo microPET evaluation revealed predominant uptake in bone with renal excretion. Compared with [18 F]-fluoride, the uptake was lower and the PET image quality was reduced. Conclusions From the present evaluation, apart from the autonomy for PET centers without an onsite cyclotron, the advantage of [68 Ga]-EDTMP over [18 F]-fluoride is not apparent and the future clinical prospect of [68 Ga]-EDTMP remains speculative.
ISSN:0969-8051
1872-9614
DOI:10.1016/j.nucmedbio.2007.03.002