Age-related changes of P2X(1) receptor mRNA in the bladder detrusor from men with and without bladder outlet obstruction

• Published in: Experimental gerontology 2007-07, Vol.42 (7), p.686-692
• Main Authors: Chua, Wendy Chen-Nee, Liu, Lu, Mansfield, Kylie J, Vaux, Kenneth J, Moore, Kate H, Millard, Richard J, Burcher, Elizabeth
• Format: Article
• Language: English
• Subjects: Aged; Aged, 80 and over; Aging - genetics; Biopsy; Humans; Male; Middle Aged; Receptors, Purinergic P2 - genetics; Receptors, Purinergic P2X; Reference Values; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - genetics; Urinary Bladder - innervation; Urinary Bladder - pathology; Urinary Bladder - physiology; Urinary Bladder - physiopathology; Urinary Bladder Neck Obstruction - genetics; Urinary Bladder Neck Obstruction - pathology; Urinary Bladder Neck Obstruction - physiopathology
• ISSN: 0531-5565
• DOI: 10.1016/j.exger.2007.02.003

The urinary bladder purinergic system is reported to change with age and with bladder dysfunction. Here, we examined the expression of purinergic P2X(1) receptors in detrusor and mucosa (urothelium+lamina propria) from male control bladder and investigated age-related P2X(1) receptor mRNA expression in control and obstructed detrusor. Biopsy specimens were obtained at cystoscopy from control patients (n=46, age range 30-86years) and patients diagnosed with outlet obstruction (n=29, 46-88years). Calponin expression (measured by RT-PCR) was similar in control and obstructed detrusor and did not change with age. Quantitative competitive RT-PCR was used to measure P2X(1) receptor and GAPDH mRNA in control and obstructed detrusor. P2X(1) receptor mRNA expression was 9-fold (p