Effects of subinhibitory concentrations of amikacin and ciprofloxacin on the hydrophobicity and adherence to epithelial cells of uropathogenic Escherichia coli strains

Abstract The effect of subinhibitory concentrations of amikacin and ciprofloxacin on the hydrophobicity and adherence to uroepithelial cells of Escherichia coli strains was investigated. The hydrophobicity of the tested strains was evaluated by the bacterial adherence to hydrocarbon–xylene test and...

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Bibliographic Details
Published in:International journal of antimicrobial agents 2007-06, Vol.29 (6), p.700-704
Main Authors: Wojnicz, Dorota, Jankowski, Stanislaw
Format: Article
Language:English
Subjects:
Adherence
Amikacin
Amikacin - pharmacology
Anti-Bacterial Agents - pharmacology
Anti-Infective Agents - pharmacology
Antibiotics. Antiinfectious agents. Antiparasitic agents
Bacterial Adhesion - drug effects
Biological and medical sciences
Ciprofloxacin
Ciprofloxacin - pharmacology
Cystitis - microbiology
Dose-Response Relationship, Drug
Epithelial Cells - microbiology
Epithelial Cells - physiology
Escherichia coli
Escherichia coli - chemistry
Escherichia coli - drug effects
Escherichia coli - isolation & purification
Escherichia coli - pathogenicity
Female
Humans
Hydrophobic and Hydrophilic Interactions
Hydrophobicity
Infectious Disease
Medical sciences
Microbial Sensitivity Tests
Pharmacology. Drug treatments
Subinhibitory concentrations
Urethra - cytology
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Summary:Abstract The effect of subinhibitory concentrations of amikacin and ciprofloxacin on the hydrophobicity and adherence to uroepithelial cells of Escherichia coli strains was investigated. The hydrophobicity of the tested strains was evaluated by the bacterial adherence to hydrocarbon–xylene test and by the salt aggregation test of ammonium sulphate. The hydrophobic character of strains exposed to 1/2 to 1/8 minimal inhibitory concentration (MIC) of amikacin and 1/2 to 1/16 MIC of ciprofloxacin was altered to a hydrophilic state. Results of the SAT also correlated with these data. Moreover, comparisons were made between the number of bacteria attached to the epithelial cells before and after exposure to 1/2, 1/4 and 1/8 MIC of antibiotics. The greatest loss of adherence capability occurred at 1/2 MIC of ciprofloxacin. In conclusion, antibiotics are often present at sub-MICs and may still be effective in reducing bacterial virulence by interfering with bacterial cell functions.
ISSN:0924-8579
1872-7913
DOI:10.1016/j.ijantimicag.2007.01.007