18S-28S rDNA is interspersed throughout heterochromatin in the millipede Enologus oxypygum (Diplopoda)

In the present study, somatic metaphase chromosomes of the millipede Enologus oxypygum (Diplopoda: Julida) were hybridized in situ with a sea urchin (Echinodermata) ribosomal probe (prR14) in order to map major RNA genes (rDNA). Chromosomal preparations were also silver stained (Ag-NOR) to evaluate...

Full description

Saved in:
Bibliographic Details
Published in:Genetica 2000, Vol.110 (2), p.151-155
Main Authors: Vitturi, R, Colomba, M S, Mandrioli, M, Pirrone, A M, Grispo, G, Barbieri, R
Format: Article
Language:English
Subjects:
Animals
Arthropods - genetics
Diplopoda
DNA, Ribosomal - genetics
Echinodermata
Enologus oxypygum
Heterochromatin - genetics
In Situ Hybridization, Fluorescence
RNA, Ribosomal, 18S - genetics
RNA, Ribosomal, 28S - genetics
rRNA 18S
rRNA 28S
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In the present study, somatic metaphase chromosomes of the millipede Enologus oxypygum (Diplopoda: Julida) were hybridized in situ with a sea urchin (Echinodermata) ribosomal probe (prR14) in order to map major RNA genes (rDNA). Chromosomal preparations were also silver stained (Ag-NOR) to evaluate the rDNA transcriptional activity. Our results indicate that RNA genes are throughout heterochromatin in eight chromosomes involving 1/4 of the total heterochromatin which, in this species, is about 67% of the total DNA. Ag-NOR and FISH patterns were not coincident.
ISSN:0016-6707
DOI:10.1023/A:1017928826304