Preparation and characterization of new challenge stocks of SIVmac32H J5 following rapid serial passage of virus in vivo

A new challenge stock of the simian immunodeficiency virus SIVmacJ5 has been produced following passage in vivo. SIVmacJ5 3/92 (J5M), was passaged serially through cynomolgus macaques (Macaca fascicularis) by intravenous inoculation of infected spleen cells isolated and prepared 14 days post-infecti...

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Bibliographic Details
Published in:Journal of medical primatology 2007-06, Vol.36 (3), p.131-142
Main Authors: Ferguson, D, Wade-Evans, A, Elsley, W, Sangster, R, Silvera, P, MacManus, S, Davis, G, Corcoran, T, Berry, N, Brown, S, Jenkins, A, Cowie, J, Sethi, M, Hull, R, Stebbings, R, Lines, J, Norley, S, Stott, E.J, Almond, N
Format: Article
Language:English
Subjects:
Amino Acid Sequence - genetics
Animals
Antibodies, Viral - analysis
Cell Line, Tumor
Cynomolgus
Cynomolgus macaque
Gene Products, env - chemistry
Gene Products, env - genetics
Humans
immunohistochemistry
in situ hybridization
Lymphoid Tissue - virology
Macaca fascicularis
Macaca fascicularis - virology
Molecular Sequence Data
pathological processes and conditions
pathology
Sequence Alignment - veterinary
Serial Passage - veterinary
Simian Acquired Immunodeficiency Syndrome - virology
Simian immunodeficiency virus
Simian Immunodeficiency Virus - growth & development
Simian Immunodeficiency Virus - isolation & purification
Simian Immunodeficiency Virus - pathogenicity
SIVmacJ5 S61MLN
Spleen - virology
Time Factors
Viral Load
Virus Replication
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Summary:A new challenge stock of the simian immunodeficiency virus SIVmacJ5 has been produced following passage in vivo. SIVmacJ5 3/92 (J5M), was passaged serially through cynomolgus macaques (Macaca fascicularis) by intravenous inoculation of infected spleen cells isolated and prepared 14 days post-infection. Two challenge stocks, SIVmacJ5 S61MLN and SIVmacJ5 S62spl, were prepared by culture of lymphoid tissue ex vivo. These virus stocks appeared better adapted for replication in M. fascicularis as demonstrated by a greater persistence of recoverable live virus from the periphery and increased pathology in lymphoid tissues 20 weeks post-challenge as detected by immunohistochemistry. Sequence analysis of the envelope gene from these stocks did not identify marked diversification of sequence as a result of this procedure. These stocks display more robust peripheral persistence and tissue pathology in cynomolgus macaques and should prove valuable analysing recombinant vaccines based upon SIVmacJ5 transgenes.
ISSN:0047-2565
1600-0684
DOI:10.1111/j.1600-0684.2007.00224.x