Direct-fed microbial PrimaLac and salinomycin modulate whole-body and intestinal oxygen consumption and intestinal mucosal cytokine production in the broiler chick

The current study investigated whole-body O2 consumption, intestinal O2 consumption, and intestinal inflammation status through mucosal cytokine production on broiler chicks fed the direct-fed microbial PrimaLac. One hundred twenty 1-d-old broiler chicks were randomly assigned to 1 of 3 experimental...

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Bibliographic Details
Published in:Poultry science 2007-06, Vol.86 (6), p.1100-1106
Main Authors: Chichlowski, M, Croom, J, McBride, B.W, Daniel, L, Davis, G, Koci, M.D
Format: Article
Language:English
Subjects:
Animal Feed
Animal Nutritional Physiological Phenomena
Animals
Anti-Bacterial Agents - pharmacology
blood gases
blood oxygen
broiler chickens
broiler feeding
cecum
cell respiration
Chickens
chicks
cytokines
Cytokines - metabolism
Diet - veterinary
Dietary Supplements
direct-fed microbials
feeds
gene expression
Gene Expression Regulation - drug effects
ileum
interleukin-1
interleukin-10
interleukin-6
intestinal mucosa
Intestinal Mucosa - drug effects
Intestinal Mucosa - metabolism
Intestines - drug effects
Lactobacillus
messenger RNA
oxygen consumption
Oxygen Consumption - drug effects
probiotics
protein synthesis
Pyrans - pharmacology
reverse transcriptase polymerase chain reaction
salinomycin
temporal variation
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Summary:The current study investigated whole-body O2 consumption, intestinal O2 consumption, and intestinal inflammation status through mucosal cytokine production on broiler chicks fed the direct-fed microbial PrimaLac. One hundred twenty 1-d-old broiler chicks were randomly assigned to 1 of 3 experimental diets: standard starter diet (control), standard starter diet with added salinomycin (SAL), and standard starter diet with added PrimaLac (DFM). Birds were housed in 2 separate rooms, the control and SAL treatments in one room and the DFM in another. Intact ileal and cecal samples were collected on d 19, 20, and 21 after measuring whole-body O2 consumption using indirect calorimetry. The O2 up-take of ileal tissue was measured using an in vitro O2 monitor. Analysis of intestinal immune status of broilers was measured by the relative differences in mRNA of both pro- and antiinflammatory cytokines: interleukin-(IL) 1beta, IL-6, and IL-10 using real-time reverse transcription-PCR. Broilers exhibited a 6 to 16% decrease in whole-body energy expenditures and up to a 47% decrease (P
ISSN:0032-5791
1525-3171
DOI:10.1093/ps/86.6.1100