Covalent Grafting of Fibronectin onto Plasma-Treated PTFE: Influence of the Conjugation Strategy on Fibronectin Biological Activity

Surface coating of synthetic materials is often considered to improve biomedical devices biocompatibility. In this study, we covalently bound fibronectin (FN) onto ammonia plasma‐treated PTFE via two crosslinkers, namely glutaric anhydride (GA) and sulfosuccinimidyl‐4‐(p‐maleimidophenyl)butyrate (su...

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Bibliographic Details
Published in:Macromolecular bioscience 2007-05, Vol.7 (5), p.738-745
Main Authors: Vallières, Karine, Petitclerc, Éric, Laroche, Gaétan
Format: Article
Language:English
Subjects:
Ammonia
Animals
Aorta - cytology
bioactivity
Biocompatible Materials
Cattle
Cell Adhesion
crosslinking
Endothelial Cells - cytology
Enzyme-Linked Immunosorbent Assay
fibronectin
Fibronectins - chemistry
Glutarates - chemistry
Plasma
polytetrafluoroethylene (PTFE)
Polytetrafluoroethylene - chemistry
Protein Conformation
Succinimides - chemistry
surface modification
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Summary:Surface coating of synthetic materials is often considered to improve biomedical devices biocompatibility. In this study, we covalently bound fibronectin (FN) onto ammonia plasma‐treated PTFE via two crosslinkers, namely glutaric anhydride (GA) and sulfosuccinimidyl‐4‐(p‐maleimidophenyl)butyrate (sulfo‐SMPB). With respect to clean PTFE, cell adhesion increased markedly on both FN grafted surfaces, although it was twice higher on PTFE‐GA‐FN than on PTFE‐SMPB‐FN. ELISA experiments performed with a polyclonal antibody revealed that the amount of FN is identical on both surfaces while monoclonal antibody specific to the RGD binding site clearly demonstrated a greater availability when FN is surface grafted through GA. These results provide evidence of a variation in protein conformation correlated with the surface conjugation strategy.
ISSN:1616-5187
1616-5195
DOI:10.1002/mabi.200600267