Wen-pi-tang-Hab-Wu-ling-san attenuates kidney ischemia/reperfusion injury in mice: A role for antioxidant enzymes and heat-shock proteins

• Published in: Journal of ethnopharmacology 2007-06, Vol.112 (2), p.333-340
• Main Authors: Seok, Y.M, Kim, J, Choi, K.C, Yoon, C.H, Boo, Y.C, Park, Y, Park, K.M
• Format: Article
• Language: English
• Subjects: Alisma plantago-aquatica; Alisma plantago-aquatica var. orientale; Animals; antioxidants; Antioxidants - metabolism; Artemisia capillaris; Atractylodes; Atractylodes macrocephala; bioassays; Biological and medical sciences; Blotting, Western; Catalase - metabolism; chemical constituents of plants; Cinnamomum aromaticum; Codonopsis; Codonopsis pilosula; Coptis; Coptis chinensis; Cytosol - drug effects; Cytosol - enzymology; Drugs, Chinese Herbal - metabolism; Epidemium koreanum; Epimedium; ethnobotany; General pharmacology; Glycyrrhiza uralensis; Heat-Shock Proteins - metabolism; herbal medicines; HSP72 Heat-Shock Proteins - metabolism; Hydrogen Peroxide - metabolism; ischemia; Kidney - pathology; kidney diseases; Kidney Diseases - drug therapy; Kidney Diseases - enzymology; Kidney Diseases - pathology; leaves; Lipid Peroxidation - drug effects; Male; Medical sciences; medicinal plants; medicinal properties; Mice; Mice, Inbred BALB C; Mitochondria - drug effects; Mitochondria - enzymology; oxidative stress; Perilla frutescens; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Pinellia ternata; plant extracts; Polyporus; Polyporus umbellatus; Poria; Poria cocos; Reperfusion Injury - drug therapy; Reperfusion Injury - enzymology; Reperfusion Injury - pathology; Rheum palmatum; rhizomes; roots; Salvia miltiorrhiza; Superoxide Dismutase - metabolism; traditional medicine
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2007.03.015

The purpose of this study was to investigate the effects of Wen-pi-tang-Hab-Wu-ling-san (WHW) extract, which has been used for treatment of renal diseases, on kidney ischemia/reperfusion (I/R) injury. Thirty minutes of bilateral renal ischemia resulted in disruption of kidney tubular epithelial cells and increased plasma creatinine levels in mice, however these effects were significantly attenuated when WHW was administered prior to I/R. WHW-administration also inhibited post-ischemic decreases of catalase, copper-zinc superoxide dismutase (CuZnSOD), and manganese superoxide dismutase (MnSOD) activity in kidney tissue, leading to decreased tissue hydrogen peroxide levels and lipid peroxidation. Post-ischemic increases of heat-shock protein (HSP)-27 and -72 expressions were greater in mouse kidneys that received WHW. In conclusion, WHW-administration reduced kidney susceptibility to I/R injury, and this reduced susceptibility was associated with greater post-ischemic activation of catalase, CuZnSOD and MnSOD, resulting in reduced hydrogen peroxide levels and lipid peroxidation, as well as higher post-ischemic expression of HSP-27 and -72.