Prevalence of stratified epithelium-specific antinuclear antibodies in 138 patients with lichen planus

• Published in: Journal of the American Academy of Dermatology 2007-06, Vol.56 (6), p.974-978
• Main Authors: Parodi, Aurora, MD, Cozzani, Emanuele, MD, PhD, Massone, Cesare, MD, Rebora, Alfredo, MD, Priano, Luigi, MD, Ghigliotti, Giovanni, MD, Balbi, Paolo, MD, Rongioletti, Franco, MD, Micalizzi, Claudia, MD, Cestari, Rossella, MD, Varaldo, Giuseppe, MD, Barabino, Gianfranco, MD, Cannata, Giuseppe, MD, Drago, Francesco, MD, Moreno, Vittorio, MD, Schiazza, Luciano, MD, Muzio, Gianfranco, MD, Scaparro, Enrico, MD, Alibrandi, Bruno, MD, Bandelloni, Roberto, MD, Ciaccio, Marina, MD, Desirello, Giovanni, MD, Isola, Pier Mario, MD, Ottoboni, Stefano, MD, Rampini, Paolo, MD, Santoro, Giuseppe, MD, Sorbara, Stefania, MD, Virno, Giovanni, MD
• Format: Article
• Language: English
• Subjects: Antibodies, Antinuclear - analysis; Biological and medical sciences; Dermatology; Epidemiology; Epithelium - immunology; Female; Fluorescent Antibody Technique, Indirect; General aspects; Humans; Immunoblotting; Lichen Planus - immunology; Male; Medical sciences; Middle Aged; Prevalence; Psoriasis. Parapsoriasis. Lichen; Public health. Hygiene; Public health. Hygiene-occupational medicine
• ISSN: 0190-9622; 1097-6787
• DOI: 10.1016/j.jaad.2005.10.033

Background Antibodies to stratified epithelia characterize chronic ulcerative stomatitis, an entity that very closely resembles erosive lichen planus both clinically and histologically. These antibodies are directed against a 70-kd antigen. Objective Our aim was to verify whether antibodies to stratified epithelia are present in patients with common lichen planus. Patients and methods One hundred thirty-eight patients with various forms of lichen planus were studied. Indirect immunofluorescence was performed on both monkey esophagus and HEp2-2000 cells. Immunoblotting was done with cultured keratinocytes used as the source antigen. Results Nineteen patients had antibodies to stratified epithelia (in 9 directed against an antigen of 70 kd). Forty-eight patients had circulating antibodies detected by indirect immunofluorescence on both monkey esophagus and HEp2-2000 cells (in 7 directed against an antigen of 70 kd). Indirect immunofluorescence was positive only on HEp2-2000 cells in 21 patients. Indirect immunofluorescence was negative in 50 patients on both HEp2-2000 cells and monkey esophagus. None of the last 71 patients had antibodies directed to an antigen of 70 kd. Limitations This is a serological study; results from direct immunofluorescence studies would be interesting. Conclusion Antibodies to stratified epithelia directed to an antigen of 70 kd are not exclusive to chronic ulcerative stomatitis, but are also present in some patients with lichen planus.