Microfluidic conductimetric bioreactor

A microfluidic conductimetric bioreactor has been developed. Enzyme was immobilized in the microfluidic channel on poly-dimethylsiloxane (PDMS) surface via covalent binding method. The detection unit consisted of two gold electrodes and a laboratory-built conductimetric transducer to monitor the inc...

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Published in:Biosensors & bioelectronics 2007-06, Vol.22 (12), p.3064-3071
Main Authors: Limbut, Warakorn, Loyprasert, Suchera, Thammakhet, Chongdee, Thavarungkul, Panote, Tuantranont, Adisorn, Asawatreratanakul, Punnee, Limsakul, Chusak, Wongkittisuksa, Booncharoen, Kanatharana, Proespichaya
Format: Article
Language:English
Subjects:
Biological and medical sciences
Bioreactor
Bioreactors
Biotechnology
Conductimetric
Enzymes, Immobilized - chemistry
Fundamental and applied biological sciences. Psychology
Humans
Immobilization
Microfluidic
Microfluidic Analytical Techniques - instrumentation
Microfluidic Analytical Techniques - methods
Urea
Urea - blood
Urease
Urease - chemistry
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creator Limbut, Warakorn
Loyprasert, Suchera
Thammakhet, Chongdee
Thavarungkul, Panote
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Asawatreratanakul, Punnee
Limsakul, Chusak
Wongkittisuksa, Booncharoen
Kanatharana, Proespichaya
description A microfluidic conductimetric bioreactor has been developed. Enzyme was immobilized in the microfluidic channel on poly-dimethylsiloxane (PDMS) surface via covalent binding method. The detection unit consisted of two gold electrodes and a laboratory-built conductimetric transducer to monitor the increase in the conductivity of the solution due to the change of the charges generated by the enzyme-substrate catalytic reaction. Urea–urease was used as a representative analyte-enzyme system. Under optimum conditions urea could be determined with a detection limit of 0.09mM and linearity in the range of 0.1–10mM (r=0.9944). The immobilized urease on the microchannel chip provided good stability (>30 days of operation time) and good repeatability with an R.S.D. lower than 2.3%. Good agreement was obtained when urea concentrations of human serum samples determined by the microfluidic flow injection conductimetric bioreactor system were compared to those obtained using the Berthelot reaction (P
doi_str_mv 10.1016/j.bios.2007.01.001
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source Elsevier
subjects Biological and medical sciences
Bioreactor
Bioreactors
Biotechnology
Conductimetric
Enzymes, Immobilized - chemistry
Fundamental and applied biological sciences. Psychology
Humans
Immobilization
Microfluidic
Microfluidic Analytical Techniques - instrumentation
Microfluidic Analytical Techniques - methods
Urea
Urea - blood
Urease
Urease - chemistry
title Microfluidic conductimetric bioreactor
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