Soluble adhesion molecules (sICAM-1, sL-Selectin, sE-Selectin, sCD44) in healthy allogenic peripheral stem-cell donors primed with recombinant G-CSF

• Published in: Cytotherapy (Oxford, England) England), 2000-04, Vol.2 (4), p.259-265
• Main Authors: Arslan, Ö., Akan, H., Arat, M., Dalva, K., Özcan, M., Gürman, G., Ilhan, O., Konuk, N., Beksaç, M., Uysal, A., Koç, H.
• Format: Article
• Language: English
• Subjects: Adult; allogeneic stem cell transplantation; Antigens, CD34 - analysis; Antigens, CD34 - immunology; Blood Donors; E-Selectin - blood; engraftment; Enzyme-Linked Immunosorbent Assay; Female; Graft vs Host Disease; Granulocyte Colony-Stimulating Factor - pharmacology; Humans; Hyaluronan Receptors - blood; Intercellular Adhesion Molecule-1 - blood; L-Selectin - blood; Leukapheresis; Male; Middle Aged; normal donors; Recombinant Proteins; Solubility; soluble adhesion molecules; stem cell mobilization; Stem Cell Transplantation - methods; Stem Cells - cytology; Stem Cells - drug effects; Time Factors; Transplantation, Homologous
• ISSN: 1465-3249; 1477-2566
• DOI: 10.1080/146532400539198

We analysed the effects of rhG-CSF (Amgen-Roche, USA) on serum changes of four soluble adhesion molecules (SAM) (sICAM-1, sL-Selectin, sE-Selectin and sCD44) in healthy peripheral allogeneic stem-cell transplantation donors and their correlation with acute GvHD and effect on engraftment kinetics. Serum SAM of 15 consecutive healthy HLA identical-sibling donors (median age 30 years, male:female ratio, 7:8) were monitored using a commercial ELISA Kit (Bender Med, Austria) prior to, on the day of first apheresis and 24h after the cessation of rhG-CSF (10μg/kg/day s.c. on 5 days) administration. Leukapheresis was started on the fifth day of rhG-CSF administration, using a continuous-flow blood separator (Cobe Spectra, COBE BCT, Inc, Lakewood, CO). Apheresis cycles were continued daily until a target of 4.0×106 CD34+ cells/kg was reached. The results indicate a steady rise of sL-Selectin, sE-Selectin, and sCD44, but not of sICAM-1. Median number of mononuclear cells (MNC) and CD34+ cells transfused were 7.7×108/kg and 6.0×106/kg, respectively. There was a near-significant correlation between the sL-Selectin levels and CD34+ cell yield (r = 0.49, 0.06). Median granulocyte and platelet engraftment days were 11 (10–18) and 12 (9–33), respectively. There was a significant inverse correlation between the CD34+ cell dose and granulocyte levels (r = -0.68, p = 0.022), but not for platelet engraftment. The only correlation between SAM levels and engraftment was for sICAM-1 levels. Increasing sICAM-1 levels were a sign of prolonged neutropenia (r = 0.72, p = 0.011). No correlation between the apheresis day serum levels of adhesion molecules and acute GvHD was documented. Analysis of sICAM-1, sL-Selectin, sE-Selectin and sCD44 levels during allogeneic PBSC apheresis did not reveal any significant effect on engraftment and GvHD, except the correlation of sL-Selectin levels and collected CD34+ cells. More research and data about the role of not only SAM levels, but also antigenic expression of SAM are required to enlighten leukocyte–endothelial cell interactions and egress of stem cells during G-CSF administration.