Whitespotted puffer Arothron hispidus, a new host for lymphocystis in Qingdao Aquarium of China

In April 2004 white nodular lesions were found on the fins of whitespotted puffer Arothron hispidus (Linnaeus). Diagnostic studies were carried out to confirm the disease using light and electron microscopy, histochemical methods and PCR. The results revealed that the nodules were composed of giant...

Full description

Saved in:
Bibliographic Details
Published in:Diseases of aquatic organisms 2007-03, Vol.75 (1), p.23-28
Main Authors: SHENG, Xiu-Zhen, ZHAN, Wen-Bin, YUE WANG
Format: Article
Language:English
Subjects:
Animal viral diseases
Animals
Arothron hispidus
Biological and medical sciences
Capsid Proteins - genetics
China
DNA Primers - chemistry
DNA Virus Infections - pathology
DNA Virus Infections - veterinary
DNA, Viral - analysis
Fish Diseases - pathology
Fish Diseases - virology
Infectious diseases
Iridoviridae - genetics
Iridoviridae - isolation & purification
Iridoviridae - pathogenicity
Lymphocystis disease virus
Marine
Medical sciences
Paralichthys olivaceus
Pleuronectiformes
Polymerase Chain Reaction
Tetraodontiformes - virology
Viral diseases
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In April 2004 white nodular lesions were found on the fins of whitespotted puffer Arothron hispidus (Linnaeus). Diagnostic studies were carried out to confirm the disease using light and electron microscopy, histochemical methods and PCR. The results revealed that the nodules were composed of giant cells up to 400 microm in diameter. These cells were surrounded by a periodic acid-Schiff (PAS)-positive hyaline capsule containing dot-shaped, Feulgen-positive inclusion bodies in the cytoplasm and an irregular nucleus. Numerous virus particles 200 nm in diameter and with hexagonal profiles were observed in the cytoplasm. These features were consistent with those of lymphocystis disease. Additionally, based on the gene sequences of major capsid protein (MCP) of lymphocystis disease virus (LCDV) from Japanese flounder Paralichthys olivaceus, 2 pair primers were designed; after a nested PCR was performed for detection of LCDV in A. hispidus, a positive amplified product was obtained showing the presence of LCDV. Therefore, the white nodules were the lymphocystis lesions caused by LCDV infection and A. hispidus was demonstrated to be a new host for LCDV.
ISSN:0177-5103
1616-1580
DOI:10.3354/dao075023