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Whitespotted puffer Arothron hispidus, a new host for lymphocystis in Qingdao Aquarium of China
In April 2004 white nodular lesions were found on the fins of whitespotted puffer Arothron hispidus (Linnaeus). Diagnostic studies were carried out to confirm the disease using light and electron microscopy, histochemical methods and PCR. The results revealed that the nodules were composed of giant...
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| Published in: | Diseases of aquatic organisms 2007-03, Vol.75 (1), p.23-28 |
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| container_title | Diseases of aquatic organisms |
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| creator | SHENG, Xiu-Zhen ZHAN, Wen-Bin YUE WANG |
| description | In April 2004 white nodular lesions were found on the fins of whitespotted puffer Arothron hispidus (Linnaeus). Diagnostic studies were carried out to confirm the disease using light and electron microscopy, histochemical methods and PCR. The results revealed that the nodules were composed of giant cells up to 400 microm in diameter. These cells were surrounded by a periodic acid-Schiff (PAS)-positive hyaline capsule containing dot-shaped, Feulgen-positive inclusion bodies in the cytoplasm and an irregular nucleus. Numerous virus particles 200 nm in diameter and with hexagonal profiles were observed in the cytoplasm. These features were consistent with those of lymphocystis disease. Additionally, based on the gene sequences of major capsid protein (MCP) of lymphocystis disease virus (LCDV) from Japanese flounder Paralichthys olivaceus, 2 pair primers were designed; after a nested PCR was performed for detection of LCDV in A. hispidus, a positive amplified product was obtained showing the presence of LCDV. Therefore, the white nodules were the lymphocystis lesions caused by LCDV infection and A. hispidus was demonstrated to be a new host for LCDV. |
| doi_str_mv | 10.3354/dao075023 |
| format | article |
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Diagnostic studies were carried out to confirm the disease using light and electron microscopy, histochemical methods and PCR. The results revealed that the nodules were composed of giant cells up to 400 microm in diameter. These cells were surrounded by a periodic acid-Schiff (PAS)-positive hyaline capsule containing dot-shaped, Feulgen-positive inclusion bodies in the cytoplasm and an irregular nucleus. Numerous virus particles 200 nm in diameter and with hexagonal profiles were observed in the cytoplasm. These features were consistent with those of lymphocystis disease. Additionally, based on the gene sequences of major capsid protein (MCP) of lymphocystis disease virus (LCDV) from Japanese flounder Paralichthys olivaceus, 2 pair primers were designed; after a nested PCR was performed for detection of LCDV in A. hispidus, a positive amplified product was obtained showing the presence of LCDV. 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ZHAN, Wen-Bin ; YUE WANG</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c379t-b060a68e1c9fb7325ad6d9b8113b499c9d6dc7c4736ed14dad129e5bbc5657873</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animal viral diseases</topic><topic>Animals</topic><topic>Arothron hispidus</topic><topic>Biological and medical sciences</topic><topic>Capsid Proteins - genetics</topic><topic>China</topic><topic>DNA Primers - chemistry</topic><topic>DNA Virus Infections - pathology</topic><topic>DNA Virus Infections - veterinary</topic><topic>DNA, Viral - analysis</topic><topic>Fish Diseases - pathology</topic><topic>Fish Diseases - virology</topic><topic>Infectious diseases</topic><topic>Iridoviridae - genetics</topic><topic>Iridoviridae - isolation & purification</topic><topic>Iridoviridae - pathogenicity</topic><topic>Lymphocystis disease virus</topic><topic>Marine</topic><topic>Medical sciences</topic><topic>Paralichthys olivaceus</topic><topic>Pleuronectiformes</topic><topic>Polymerase Chain Reaction</topic><topic>Tetraodontiformes - virology</topic><topic>Viral diseases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SHENG, Xiu-Zhen</creatorcontrib><creatorcontrib>ZHAN, Wen-Bin</creatorcontrib><creatorcontrib>YUE WANG</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Oceanic Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><jtitle>Diseases of aquatic organisms</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SHENG, Xiu-Zhen</au><au>ZHAN, Wen-Bin</au><au>YUE WANG</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Whitespotted puffer Arothron hispidus, a new host for lymphocystis in Qingdao Aquarium of China</atitle><jtitle>Diseases of aquatic organisms</jtitle><addtitle>Dis Aquat Organ</addtitle><date>2007-03-29</date><risdate>2007</risdate><volume>75</volume><issue>1</issue><spage>23</spage><epage>28</epage><pages>23-28</pages><issn>0177-5103</issn><eissn>1616-1580</eissn><coden>DAOREO</coden><abstract>In April 2004 white nodular lesions were found on the fins of whitespotted puffer Arothron hispidus (Linnaeus). Diagnostic studies were carried out to confirm the disease using light and electron microscopy, histochemical methods and PCR. The results revealed that the nodules were composed of giant cells up to 400 microm in diameter. These cells were surrounded by a periodic acid-Schiff (PAS)-positive hyaline capsule containing dot-shaped, Feulgen-positive inclusion bodies in the cytoplasm and an irregular nucleus. Numerous virus particles 200 nm in diameter and with hexagonal profiles were observed in the cytoplasm. These features were consistent with those of lymphocystis disease. Additionally, based on the gene sequences of major capsid protein (MCP) of lymphocystis disease virus (LCDV) from Japanese flounder Paralichthys olivaceus, 2 pair primers were designed; after a nested PCR was performed for detection of LCDV in A. hispidus, a positive amplified product was obtained showing the presence of LCDV. Therefore, the white nodules were the lymphocystis lesions caused by LCDV infection and A. hispidus was demonstrated to be a new host for LCDV.</abstract><cop>Oldendorf</cop><pub>Inter-Research</pub><pmid>17523540</pmid><doi>10.3354/dao075023</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Diseases of aquatic organisms, 2007-03, Vol.75 (1), p.23-28 |
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| language | eng |
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| source | Freely Accessible Science Journals |
| subjects | Animal viral diseases Animals Arothron hispidus Biological and medical sciences Capsid Proteins - genetics China DNA Primers - chemistry DNA Virus Infections - pathology DNA Virus Infections - veterinary DNA, Viral - analysis Fish Diseases - pathology Fish Diseases - virology Infectious diseases Iridoviridae - genetics Iridoviridae - isolation & purification Iridoviridae - pathogenicity Lymphocystis disease virus Marine Medical sciences Paralichthys olivaceus Pleuronectiformes Polymerase Chain Reaction Tetraodontiformes - virology Viral diseases |
| title | Whitespotted puffer Arothron hispidus, a new host for lymphocystis in Qingdao Aquarium of China |
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