Some efficient methods to correct confocal images for easy interpretation

In this paper we have explained some efficient methods to correct artefacts in confocal laser beam scanning microscope (CLSM) images. The main aim is to enhance object features such that they become clearly visible for interactive evaluation and to reduce the overall noise so that the automatic segm...

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Bibliographic Details
Published in:Micron (Oxford, England : 1993) England : 1993), 2001-06, Vol.32 (4), p.363-370
Main Authors: Umesh Adiga, P.S, Chaudhuri, B.B
Format: Article
Language:English
Subjects:
Carcinoma - pathology
Humans
Image Processing, Computer-Assisted - methods
Intensity
Interpolation
Male
Microscopy, Confocal
Morphing
Noise
Photobleaching
Prostatic Neoplasms - pathology
Restoration
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Summary:In this paper we have explained some efficient methods to correct artefacts in confocal laser beam scanning microscope (CLSM) images. The main aim is to enhance object features such that they become clearly visible for interactive evaluation and to reduce the overall noise so that the automatic segmentation and feature measurement can be done easily. A simple automatic-thresholding technique, and a straightforward method to restore the light intensity along the depth of the image stack are proposed. Another problem associated with the CLSM is the non-isotropic resolution. We have presented an interpolation technique based on XOR contouring and morphing to virtually insert the image slices in the image stack for improving the axial resolution. This interpolation technique has the merits of both contour- and intensity-based interpolations. Results of application of these methods on CLSM data are shown.
ISSN:0968-4328
1878-4291
DOI:10.1016/S0968-4328(00)00046-9