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Effects of Acute Graded Reductions in Testicular Blood Flow on Testicular Morphology in the Adult Rat
The effect of moderate reductions in testicular blood flow has not been studied systematically. The aim of this study was, therefore, to examine the effects of different degrees of blood flow reduction on testicular morphology and to determine how much flow can be reduced before damage occurs. The s...
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| Published in: | Biology of reproduction 2001-01, Vol.64 (1), p.13-20 |
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| Language: | English |
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| container_title | Biology of reproduction |
| container_volume | 64 |
| creator | Bergh, A Collin, O Lissbrant, E |
| description | The effect of moderate reductions in testicular blood flow has not been studied systematically. The aim of this study was,
therefore, to examine the effects of different degrees of blood flow reduction on testicular morphology and to determine how
much flow can be reduced before damage occurs. The subcapsular testicular artery was partially ligated in the left testes
of adult rats. Testicular blood flow was measured before, immediately after, and 5 h after the ligation using laser Doppler
flowmetry. After 5 h of partial ligation, the testes were removed, and their morphology was examined and related to the degree
of blood flow reduction. The number of in situ end-labeled- or TUNEL-positive (i.e., dying) germ cells and the volume density
of intravascular polymorphonuclear (PMN) leukocytes were measured. When flow was reduced to approximately 70% or less of its
pretreatment value, a dose-related increase in the number of dying spermatogonia and early spermatocytes was seen. The PMN
leukocytes accumulated in testicular blood vessels after partial ligation, and the maximum number was observed in testes where
flow was reduced by approximately 50% of the pretreatment value. In conclusion, early stages of spermatogenesis are sensitive
to a moderate, acute reduction in blood flow. Discrete reductions in flow may, therefore, have a large impact on sperm production. |
| doi_str_mv | 10.1095/biolreprod64.1.13 |
| format | article |
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therefore, to examine the effects of different degrees of blood flow reduction on testicular morphology and to determine how
much flow can be reduced before damage occurs. The subcapsular testicular artery was partially ligated in the left testes
of adult rats. Testicular blood flow was measured before, immediately after, and 5 h after the ligation using laser Doppler
flowmetry. After 5 h of partial ligation, the testes were removed, and their morphology was examined and related to the degree
of blood flow reduction. The number of in situ end-labeled- or TUNEL-positive (i.e., dying) germ cells and the volume density
of intravascular polymorphonuclear (PMN) leukocytes were measured. When flow was reduced to approximately 70% or less of its
pretreatment value, a dose-related increase in the number of dying spermatogonia and early spermatocytes was seen. The PMN
leukocytes accumulated in testicular blood vessels after partial ligation, and the maximum number was observed in testes where
flow was reduced by approximately 50% of the pretreatment value. In conclusion, early stages of spermatogenesis are sensitive
to a moderate, acute reduction in blood flow. Discrete reductions in flow may, therefore, have a large impact on sperm production.</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod64.1.13</identifier><identifier>PMID: 11133653</identifier><language>eng</language><publisher>United States: Society for the Study of Reproduction</publisher><subject>Animals ; Apoptosis ; Arteries - surgery ; Blood Flow Velocity ; Cell Count ; In Situ Nick-End Labeling ; Ischemia - etiology ; Ischemia - pathology ; Ischemia - physiopathology ; Kinetics ; Laser-Doppler Flowmetry ; Leukocyte Count ; Ligation ; Male ; Microcirculation - pathology ; Microcirculation - physiology ; Neutrophils ; Organ Size ; Rats ; Rats, Sprague-Dawley ; Spermatogenesis ; Spermatozoa - physiology ; Testis - blood supply ; Testis - pathology</subject><ispartof>Biology of reproduction, 2001-01, Vol.64 (1), p.13-20</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11133653$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bergh, A</creatorcontrib><creatorcontrib>Collin, O</creatorcontrib><creatorcontrib>Lissbrant, E</creatorcontrib><title>Effects of Acute Graded Reductions in Testicular Blood Flow on Testicular Morphology in the Adult Rat</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>The effect of moderate reductions in testicular blood flow has not been studied systematically. The aim of this study was,
therefore, to examine the effects of different degrees of blood flow reduction on testicular morphology and to determine how
much flow can be reduced before damage occurs. The subcapsular testicular artery was partially ligated in the left testes
of adult rats. Testicular blood flow was measured before, immediately after, and 5 h after the ligation using laser Doppler
flowmetry. After 5 h of partial ligation, the testes were removed, and their morphology was examined and related to the degree
of blood flow reduction. The number of in situ end-labeled- or TUNEL-positive (i.e., dying) germ cells and the volume density
of intravascular polymorphonuclear (PMN) leukocytes were measured. When flow was reduced to approximately 70% or less of its
pretreatment value, a dose-related increase in the number of dying spermatogonia and early spermatocytes was seen. The PMN
leukocytes accumulated in testicular blood vessels after partial ligation, and the maximum number was observed in testes where
flow was reduced by approximately 50% of the pretreatment value. In conclusion, early stages of spermatogenesis are sensitive
to a moderate, acute reduction in blood flow. Discrete reductions in flow may, therefore, have a large impact on sperm production.</description><subject>Animals</subject><subject>Apoptosis</subject><subject>Arteries - surgery</subject><subject>Blood Flow Velocity</subject><subject>Cell Count</subject><subject>In Situ Nick-End Labeling</subject><subject>Ischemia - etiology</subject><subject>Ischemia - pathology</subject><subject>Ischemia - physiopathology</subject><subject>Kinetics</subject><subject>Laser-Doppler Flowmetry</subject><subject>Leukocyte Count</subject><subject>Ligation</subject><subject>Male</subject><subject>Microcirculation - pathology</subject><subject>Microcirculation - physiology</subject><subject>Neutrophils</subject><subject>Organ Size</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Spermatogenesis</subject><subject>Spermatozoa - physiology</subject><subject>Testis - blood supply</subject><subject>Testis - pathology</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><recordid>eNpV0M1OwzAMAOAIgdgYPAAXlAPi1hE3P2uPY9oG0hDSNM5VlqRrUbqMpFW1tydo48DJlv3ZsozQPZAxkJw_b2tnvTl4pwUbwxjoBRoCT_NkkorsEg0JISKhVNABugnhixBgNKXXaAAAsczpEJl5WRrVBuxKPFVda_DSS200XhvdqbZ2-4DrPd6Y0Naqs9LjF-ucxgvreuz-Nd6dP1TOut3xd6KtDJ7qzrZ4LdtbdFVKG8zdOY7Q52K-mb0mq4_l22y6SqqUijahuVCGSzNRkGvGgWrOY0Y5kyXkDGQmS6JNnitJiRZpqbQGEExrBpxuKR2hp9Pe-JPvLp5WNHVQxlq5N64LxYRwlmUZi_DhDLttY3Rx8HUj_bH4e0wEjydQ1buqr70pQiOtjZwWfd8LVkAR6Q9g_3Qt</recordid><startdate>20010101</startdate><enddate>20010101</enddate><creator>Bergh, A</creator><creator>Collin, O</creator><creator>Lissbrant, E</creator><general>Society for the Study of Reproduction</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20010101</creationdate><title>Effects of Acute Graded Reductions in Testicular Blood Flow on Testicular Morphology in the Adult Rat</title><author>Bergh, A ; Collin, O ; Lissbrant, E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h236t-396ce5ae7c19d4513d5519d354af1941a8af0de99ca30d62fcdd1164dd4153b33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Apoptosis</topic><topic>Arteries - surgery</topic><topic>Blood Flow Velocity</topic><topic>Cell Count</topic><topic>In Situ Nick-End Labeling</topic><topic>Ischemia - etiology</topic><topic>Ischemia - pathology</topic><topic>Ischemia - physiopathology</topic><topic>Kinetics</topic><topic>Laser-Doppler Flowmetry</topic><topic>Leukocyte Count</topic><topic>Ligation</topic><topic>Male</topic><topic>Microcirculation - pathology</topic><topic>Microcirculation - physiology</topic><topic>Neutrophils</topic><topic>Organ Size</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Spermatogenesis</topic><topic>Spermatozoa - physiology</topic><topic>Testis - blood supply</topic><topic>Testis - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bergh, A</creatorcontrib><creatorcontrib>Collin, O</creatorcontrib><creatorcontrib>Lissbrant, E</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bergh, A</au><au>Collin, O</au><au>Lissbrant, E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Acute Graded Reductions in Testicular Blood Flow on Testicular Morphology in the Adult Rat</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>2001-01-01</date><risdate>2001</risdate><volume>64</volume><issue>1</issue><spage>13</spage><epage>20</epage><pages>13-20</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><abstract>The effect of moderate reductions in testicular blood flow has not been studied systematically. The aim of this study was,
therefore, to examine the effects of different degrees of blood flow reduction on testicular morphology and to determine how
much flow can be reduced before damage occurs. The subcapsular testicular artery was partially ligated in the left testes
of adult rats. Testicular blood flow was measured before, immediately after, and 5 h after the ligation using laser Doppler
flowmetry. After 5 h of partial ligation, the testes were removed, and their morphology was examined and related to the degree
of blood flow reduction. The number of in situ end-labeled- or TUNEL-positive (i.e., dying) germ cells and the volume density
of intravascular polymorphonuclear (PMN) leukocytes were measured. When flow was reduced to approximately 70% or less of its
pretreatment value, a dose-related increase in the number of dying spermatogonia and early spermatocytes was seen. The PMN
leukocytes accumulated in testicular blood vessels after partial ligation, and the maximum number was observed in testes where
flow was reduced by approximately 50% of the pretreatment value. In conclusion, early stages of spermatogenesis are sensitive
to a moderate, acute reduction in blood flow. Discrete reductions in flow may, therefore, have a large impact on sperm production.</abstract><cop>United States</cop><pub>Society for the Study of Reproduction</pub><pmid>11133653</pmid><doi>10.1095/biolreprod64.1.13</doi><tpages>8</tpages></addata></record> |
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| ispartof | Biology of reproduction, 2001-01, Vol.64 (1), p.13-20 |
| issn | 0006-3363 1529-7268 |
| language | eng |
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| source | Oxford Journals Online |
| subjects | Animals Apoptosis Arteries - surgery Blood Flow Velocity Cell Count In Situ Nick-End Labeling Ischemia - etiology Ischemia - pathology Ischemia - physiopathology Kinetics Laser-Doppler Flowmetry Leukocyte Count Ligation Male Microcirculation - pathology Microcirculation - physiology Neutrophils Organ Size Rats Rats, Sprague-Dawley Spermatogenesis Spermatozoa - physiology Testis - blood supply Testis - pathology |
| title | Effects of Acute Graded Reductions in Testicular Blood Flow on Testicular Morphology in the Adult Rat |
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