Chitosan oligosaccharide (COS) inhibits LPS-induced inflammatory effects in RAW 264.7 macrophage cells

• Published in: Biochemical and biophysical research communications 2007-07, Vol.358 (3), p.954-959
• Main Authors: Yoon, Hyun Joong, Moon, Myoung E., Park, Haeng Soon, Im, Suhn Young, Kim, Young Ho
• Format: Article
• Language: English
• Subjects: Animals; Anti-Inflammatory Agents - pharmacology; Cell Line; Chitosan - chemistry; Chitosan oligosaccharide (COS); Inflammation; Interleukin-6 (IL-6); Interleukin-6 - metabolism; Lipopolysaccharide (LPS); Lipopolysaccharides - metabolism; Macrophages - metabolism; Mice; Nitric oxide (NO); Nitric Oxide - metabolism; Nitrites - chemistry; Oligosaccharides - metabolism; Oligosaccharides - physiology; Temperature; Time Factors; Tumor Necrosis Factor-alpha - metabolism; Tumor necrosis factor-α (TNF-α)
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2007.05.042

The focus of this study was to clarify the relation between the nitric oxide (NO) production and cytokine expression including tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), and also investigated the effect of COS on LPS stimuli from RAW 264.7 cell. The lipopolysaccharide (LPS) of Gram-negative bacteria induces the expression of cytokines and potent inducers of inflammatory cytokines such as TNF-α and IL-6. In this experiment, upon stimulation with increasing concentrations of chitosan, the LPS-stimulated TNF-α and IL-6 secretion was significantly recovered within the incubation media of RAW 264.7 cells. Consistently, RT-PCR with mRNA and Western blot with anti-cytokine antiserum including TNF-α and IL-6 showed that the amount of TNF-α and IL-6 secretion in the incubation media recovered with the concentration of chitosan. The LPS-stimulated NO secretion was significantly recovered within the 6 h and 12 h incubation media of RAW 264.7 cells, too. The recovery effect of chitosan on IL-6 and NO secretion may be induced via the stimulus of TNF-α in RAW 264.7 cell. These results once again suggest that chitosan oligosaccharide may have the anti-inflammatory effect via the stimulus of TNF-α in the LPS-stimulated inflammation in RAW 264.7 cells.