Measurement of Homogeneous Kinase Activity for Cell Lysates Based on the Aggregation of Gold Nanoparticles

When the charge sticks. A kinase activity screening system based on the aggregation of gold nanoparticles induced by cationic substrate peptides as coagulants has been constructed. The system can conduct and monitor kinase reactions under homogeneous conditions and has been successfully applied to t...

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Bibliographic Details
Published in:Chembiochem : a European journal of chemical biology 2007-05, Vol.8 (8), p.875-879
Main Authors: Oishi, Jun, Asami, Yoji, Mori, Takeshi, Kang, Jeong-Hun, Tanabe, Miharu, Niidome, Takuro, Katayama, Yoshiki
Format: Article
Language:English
Subjects:
aggregation
Cell Extracts - chemistry
Cell Line
Enzyme Activation
enzymes
gold
Gold - chemistry
high-throughput screening
Metal Nanoparticles - chemistry
Protein Kinases - chemical synthesis
Protein Kinases - chemistry
Surface Properties
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Summary:When the charge sticks. A kinase activity screening system based on the aggregation of gold nanoparticles induced by cationic substrate peptides as coagulants has been constructed. The system can conduct and monitor kinase reactions under homogeneous conditions and has been successfully applied to the kinase assay of cell lysates.
ISSN:1439-4227
1439-7633
DOI:10.1002/cbic.200700086