Human Peripheral Blood Dendritic Cells and Monocyte Subsets Display Similar Chemokine Receptor Expression Profiles with Differential Migratory Responses

Human antigen presenting cells (APC) found in peripheral blood are considered to be precursors that have been released from the bone marrow and are in transit to the peripheral tissues. These APC populations include myeloid dendritic cells (mDC), plasmacytoid DC (pDC) and monocytes (Mo). To assign s...

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Bibliographic Details
Published in:Scandinavian journal of immunology 2007-06, Vol.65 (6), p.514-524
Main Authors: Cravens, P.D, Hayashida, K, Davis, L.S, Nanki, T, Lipsky, P.E
Format: Article
Language:English
Subjects:
Antigen-Presenting Cells - classification
Antigen-Presenting Cells - immunology
Antigens, CD - analysis
Antigens, CD - blood
Antigens, Differentiation, Myelomonocytic
Biomarkers
Cell Lineage
Cell Movement - immunology
Cell Separation
Dendritic Cells - immunology
Flow Cytometry
Humans
Immunophenotyping
Lipopolysaccharide Receptors
Monocytes - immunology
Plasma Cells - immunology
Receptors, Chemokine - metabolism
Sialic Acid Binding Ig-like Lectin 3
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Summary:Human antigen presenting cells (APC) found in peripheral blood are considered to be precursors that have been released from the bone marrow and are in transit to the peripheral tissues. These APC populations include myeloid dendritic cells (mDC), plasmacytoid DC (pDC) and monocytes (Mo). To assign specialized functional roles and stages of development for APCs, CD33 expressing APC subsets were examined for their capacity to respond to chemokines. Three major CD33⁺ subsets including CD33brightCD14bright Mo, CD33brightCD14⁻ CD11c⁺ mDC and CD33dimCD14⁻ pDC were present. Dendritic cells subsets and Mo expressed low levels of CC and CXC receptors, but distinctive chemokine receptor expression profiles were not observed. The percentage of cells expressing a particular chemokine receptor varied from donor to donor and over time in the same donor. Myeloid DC and Mo but not pDC migrated toward CXCL12 in a concentration dependent manner. Monocytes and pDC, but not myeloid DC, were attracted by high concentrations of CXCL10. All CD33⁺ subsets migrated in a concentration dependent manner toward CCL19, but responded less robustly to CCL21. CCL20 was not chemoattractant for any population. Despite the finding that APC did not exhibit unique surface chemokine receptor expression patterns, they exhibited differential migration to CXCL12, CXCL10 and CCL21 but not to CCL20 or CCL19.
ISSN:0300-9475
1365-3083
DOI:10.1111/j.1365-3083.2007.01933.x