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Characterization of necrosis and ethylene-inducing proteins (NEP) in the basidiomycete Moniliophthora perniciosa, the causal agent of witches' broom in Theobroma cacao
The hemibiotrophic basidiomycete Moniliophthora perniciosa causes witches' broom disease of Theobroma cacao. Analysis of the M. perniciosa draft genome led to the identification of three putative genes encoding necrosis and ethylene-inducing proteins (MpNEPs), which are apparently located on th...
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| Published in: | Mycological research 2007-04, Vol.111 (4), p.443-455 |
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| Main Authors: | , , , , , , , , , , , , , , |
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| container_title | Mycological research |
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| creator | Garcia, Odalys Macedo, Joci A.N. Tibúrcio, Ricardo Zaparoli, Gustavo Rincones, Johana Bittencourt, Livia M.C. Ceita, Geruza O. Micheli, Fabienne Gesteira, Abelmon Mariano, Andréa C. Schiavinato, Marlene A. Medrano, Francisco J. Meinhardt, Lyndel W. Pereira, Gonçalo A.G. Cascardo, Júlio C.M. |
| description | The hemibiotrophic basidiomycete
Moniliophthora perniciosa causes witches' broom disease of
Theobroma cacao. Analysis of the
M. perniciosa draft genome led to the identification of three putative genes encoding necrosis and ethylene-inducing proteins (MpNEPs), which are apparently located on the same chromosome. MpNEP1 and 2 have highly similar sequences and are able to induce necrosis and ethylene emission in tobacco and cacao leaves. MpNEP1 is expressed in both biotrophic and saprotrophic mycelia, the protein behaves as an oligomer in solution and is very sensitive to temperature. MpNEP2 is expressed mainly in biotrophic mycelia, is present as a monomer in solution at low concentrations ( |
| doi_str_mv | 10.1016/j.mycres.2007.01.017 |
| format | article |
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Moniliophthora perniciosa causes witches' broom disease of
Theobroma cacao. Analysis of the
M. perniciosa draft genome led to the identification of three putative genes encoding necrosis and ethylene-inducing proteins (MpNEPs), which are apparently located on the same chromosome. MpNEP1 and 2 have highly similar sequences and are able to induce necrosis and ethylene emission in tobacco and cacao leaves. MpNEP1 is expressed in both biotrophic and saprotrophic mycelia, the protein behaves as an oligomer in solution and is very sensitive to temperature. MpNEP2 is expressed mainly in biotrophic mycelia, is present as a monomer in solution at low concentrations (<40
μ
m) and is able to recover necrosis activity after boiling. These differences indicate that similar NEPs can have distinct physical characteristics and suggest possible complementary roles during the disease development for both proteins. This is the first report of NEP1-like proteins in a basidiomycete.</description><identifier>ISSN: 0953-7562</identifier><identifier>EISSN: 1469-8102</identifier><identifier>DOI: 10.1016/j.mycres.2007.01.017</identifier><identifier>PMID: 17512713</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Agaricales - chemistry ; Agaricales - genetics ; Amino Acid Sequence ; Basidiomycetes ; Basidiomycota ; Basidiomycota - chemistry ; Basidiomycota - genetics ; Basidiomycota - metabolism ; Cacao ; Cacao - virology ; ethylene ; Ethylenes - biosynthesis ; fungal proteins ; Fungal Proteins - genetics ; Fungal Proteins - metabolism ; gene expression regulation ; host plants ; Molecular Sequence Data ; Moniliophthora ; Moniliophthora perniciosa ; Mycelium - metabolism ; Necrosis ; Plant Diseases - microbiology ; Plant Leaves - metabolism ; plant pathogenic fungi ; Plant pathology ; recombinant fusion proteins ; Sequence Alignment ; sequence analysis ; Theobroma cacao ; WBD ; witches' broom</subject><ispartof>Mycological research, 2007-04, Vol.111 (4), p.443-455</ispartof><rights>2007 The British Mycological Society</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c415t-79f1556ea48a392b3198ad8bae1259463916fd201c842ee482cef0a75be6e24d3</citedby><cites>FETCH-LOGICAL-c415t-79f1556ea48a392b3198ad8bae1259463916fd201c842ee482cef0a75be6e24d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17512713$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Garcia, Odalys</creatorcontrib><creatorcontrib>Macedo, Joci A.N.</creatorcontrib><creatorcontrib>Tibúrcio, Ricardo</creatorcontrib><creatorcontrib>Zaparoli, Gustavo</creatorcontrib><creatorcontrib>Rincones, Johana</creatorcontrib><creatorcontrib>Bittencourt, Livia M.C.</creatorcontrib><creatorcontrib>Ceita, Geruza O.</creatorcontrib><creatorcontrib>Micheli, Fabienne</creatorcontrib><creatorcontrib>Gesteira, Abelmon</creatorcontrib><creatorcontrib>Mariano, Andréa C.</creatorcontrib><creatorcontrib>Schiavinato, Marlene A.</creatorcontrib><creatorcontrib>Medrano, Francisco J.</creatorcontrib><creatorcontrib>Meinhardt, Lyndel W.</creatorcontrib><creatorcontrib>Pereira, Gonçalo A.G.</creatorcontrib><creatorcontrib>Cascardo, Júlio C.M.</creatorcontrib><title>Characterization of necrosis and ethylene-inducing proteins (NEP) in the basidiomycete Moniliophthora perniciosa, the causal agent of witches' broom in Theobroma cacao</title><title>Mycological research</title><addtitle>Mycol Res</addtitle><description>The hemibiotrophic basidiomycete
Moniliophthora perniciosa causes witches' broom disease of
Theobroma cacao. Analysis of the
M. perniciosa draft genome led to the identification of three putative genes encoding necrosis and ethylene-inducing proteins (MpNEPs), which are apparently located on the same chromosome. MpNEP1 and 2 have highly similar sequences and are able to induce necrosis and ethylene emission in tobacco and cacao leaves. MpNEP1 is expressed in both biotrophic and saprotrophic mycelia, the protein behaves as an oligomer in solution and is very sensitive to temperature. MpNEP2 is expressed mainly in biotrophic mycelia, is present as a monomer in solution at low concentrations (<40
μ
m) and is able to recover necrosis activity after boiling. These differences indicate that similar NEPs can have distinct physical characteristics and suggest possible complementary roles during the disease development for both proteins. This is the first report of NEP1-like proteins in a basidiomycete.</description><subject>Agaricales - chemistry</subject><subject>Agaricales - genetics</subject><subject>Amino Acid Sequence</subject><subject>Basidiomycetes</subject><subject>Basidiomycota</subject><subject>Basidiomycota - chemistry</subject><subject>Basidiomycota - genetics</subject><subject>Basidiomycota - metabolism</subject><subject>Cacao</subject><subject>Cacao - virology</subject><subject>ethylene</subject><subject>Ethylenes - biosynthesis</subject><subject>fungal proteins</subject><subject>Fungal Proteins - genetics</subject><subject>Fungal Proteins - metabolism</subject><subject>gene expression regulation</subject><subject>host plants</subject><subject>Molecular Sequence Data</subject><subject>Moniliophthora</subject><subject>Moniliophthora perniciosa</subject><subject>Mycelium - metabolism</subject><subject>Necrosis</subject><subject>Plant Diseases - microbiology</subject><subject>Plant Leaves - metabolism</subject><subject>plant pathogenic fungi</subject><subject>Plant pathology</subject><subject>recombinant fusion proteins</subject><subject>Sequence Alignment</subject><subject>sequence analysis</subject><subject>Theobroma cacao</subject><subject>WBD</subject><subject>witches' broom</subject><issn>0953-7562</issn><issn>1469-8102</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNqFkduKFDEQhhtR3HH1DURz5QHsMUmf0jeCDOsB1gO4ex2q09XTtXQns0lGGV_I1zTtDHinUFAUfPXX4c-yx4KvBRf165v1fDAew1py3qy5SNHcyVairNtcCS7vZiveVkXeVLU8yx6EcMO5KIQo7mdnoqmEbESxyn5tRvBgInr6CZGcZW5gFo13gQID2zOM42FCiznZfm_IbtnOu4hkA3vx-eLrS0aWxRFZB4F6cmkpjMg-OUsTud0YR-eB7dBbMuQCvPoDG9gHmBhs0cZl4g-KZsTwnHXeuXmRvBrRpWKGxBpwD7N7A0wBH53yeXb97uJq8yG__PL-4-btZW5KUcW8aQdRVTVCqaBoZVeIVkGvOkAhq7asi1bUQy-5MKqUiKWSBgcOTdVhjbLsi_Ps2VE3HXm7xxD1TMHgNIFFtw-64UleqPa_oOSyVlXVJLA8gstPg8dB7zzN4A9acL04qW_00Um9OKm5SLG0PTnp77sZ-79NJ-sS8PQIDOA0bD0Fff0tXVZwrlSpmoV4cyQwPew7odfBEFqDPXk0UfeO_r3Dby8LvXU</recordid><startdate>20070401</startdate><enddate>20070401</enddate><creator>Garcia, Odalys</creator><creator>Macedo, Joci A.N.</creator><creator>Tibúrcio, Ricardo</creator><creator>Zaparoli, Gustavo</creator><creator>Rincones, Johana</creator><creator>Bittencourt, Livia M.C.</creator><creator>Ceita, Geruza O.</creator><creator>Micheli, Fabienne</creator><creator>Gesteira, Abelmon</creator><creator>Mariano, Andréa C.</creator><creator>Schiavinato, Marlene A.</creator><creator>Medrano, Francisco J.</creator><creator>Meinhardt, Lyndel W.</creator><creator>Pereira, Gonçalo A.G.</creator><creator>Cascardo, Júlio C.M.</creator><general>Elsevier Ltd</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20070401</creationdate><title>Characterization of necrosis and ethylene-inducing proteins (NEP) in the basidiomycete Moniliophthora perniciosa, the causal agent of witches' broom in Theobroma cacao</title><author>Garcia, Odalys ; Macedo, Joci A.N. ; Tibúrcio, Ricardo ; Zaparoli, Gustavo ; Rincones, Johana ; Bittencourt, Livia M.C. ; Ceita, Geruza O. ; Micheli, Fabienne ; Gesteira, Abelmon ; Mariano, Andréa C. ; Schiavinato, Marlene A. ; Medrano, Francisco J. ; Meinhardt, Lyndel W. ; Pereira, Gonçalo A.G. ; Cascardo, Júlio C.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c415t-79f1556ea48a392b3198ad8bae1259463916fd201c842ee482cef0a75be6e24d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Agaricales - chemistry</topic><topic>Agaricales - genetics</topic><topic>Amino Acid Sequence</topic><topic>Basidiomycetes</topic><topic>Basidiomycota</topic><topic>Basidiomycota - chemistry</topic><topic>Basidiomycota - genetics</topic><topic>Basidiomycota - metabolism</topic><topic>Cacao</topic><topic>Cacao - virology</topic><topic>ethylene</topic><topic>Ethylenes - biosynthesis</topic><topic>fungal proteins</topic><topic>Fungal Proteins - genetics</topic><topic>Fungal Proteins - metabolism</topic><topic>gene expression regulation</topic><topic>host plants</topic><topic>Molecular Sequence Data</topic><topic>Moniliophthora</topic><topic>Moniliophthora perniciosa</topic><topic>Mycelium - metabolism</topic><topic>Necrosis</topic><topic>Plant Diseases - microbiology</topic><topic>Plant Leaves - metabolism</topic><topic>plant pathogenic fungi</topic><topic>Plant pathology</topic><topic>recombinant fusion proteins</topic><topic>Sequence Alignment</topic><topic>sequence analysis</topic><topic>Theobroma cacao</topic><topic>WBD</topic><topic>witches' broom</topic><toplevel>online_resources</toplevel><creatorcontrib>Garcia, Odalys</creatorcontrib><creatorcontrib>Macedo, Joci A.N.</creatorcontrib><creatorcontrib>Tibúrcio, Ricardo</creatorcontrib><creatorcontrib>Zaparoli, Gustavo</creatorcontrib><creatorcontrib>Rincones, Johana</creatorcontrib><creatorcontrib>Bittencourt, Livia M.C.</creatorcontrib><creatorcontrib>Ceita, Geruza O.</creatorcontrib><creatorcontrib>Micheli, Fabienne</creatorcontrib><creatorcontrib>Gesteira, Abelmon</creatorcontrib><creatorcontrib>Mariano, Andréa C.</creatorcontrib><creatorcontrib>Schiavinato, Marlene A.</creatorcontrib><creatorcontrib>Medrano, Francisco J.</creatorcontrib><creatorcontrib>Meinhardt, Lyndel W.</creatorcontrib><creatorcontrib>Pereira, Gonçalo A.G.</creatorcontrib><creatorcontrib>Cascardo, Júlio C.M.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Mycological research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Garcia, Odalys</au><au>Macedo, Joci A.N.</au><au>Tibúrcio, Ricardo</au><au>Zaparoli, Gustavo</au><au>Rincones, Johana</au><au>Bittencourt, Livia M.C.</au><au>Ceita, Geruza O.</au><au>Micheli, Fabienne</au><au>Gesteira, Abelmon</au><au>Mariano, Andréa C.</au><au>Schiavinato, Marlene A.</au><au>Medrano, Francisco J.</au><au>Meinhardt, Lyndel W.</au><au>Pereira, Gonçalo A.G.</au><au>Cascardo, Júlio C.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of necrosis and ethylene-inducing proteins (NEP) in the basidiomycete Moniliophthora perniciosa, the causal agent of witches' broom in Theobroma cacao</atitle><jtitle>Mycological research</jtitle><addtitle>Mycol Res</addtitle><date>2007-04-01</date><risdate>2007</risdate><volume>111</volume><issue>4</issue><spage>443</spage><epage>455</epage><pages>443-455</pages><issn>0953-7562</issn><eissn>1469-8102</eissn><abstract>The hemibiotrophic basidiomycete
Moniliophthora perniciosa causes witches' broom disease of
Theobroma cacao. Analysis of the
M. perniciosa draft genome led to the identification of three putative genes encoding necrosis and ethylene-inducing proteins (MpNEPs), which are apparently located on the same chromosome. MpNEP1 and 2 have highly similar sequences and are able to induce necrosis and ethylene emission in tobacco and cacao leaves. MpNEP1 is expressed in both biotrophic and saprotrophic mycelia, the protein behaves as an oligomer in solution and is very sensitive to temperature. MpNEP2 is expressed mainly in biotrophic mycelia, is present as a monomer in solution at low concentrations (<40
μ
m) and is able to recover necrosis activity after boiling. These differences indicate that similar NEPs can have distinct physical characteristics and suggest possible complementary roles during the disease development for both proteins. This is the first report of NEP1-like proteins in a basidiomycete.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>17512713</pmid><doi>10.1016/j.mycres.2007.01.017</doi><tpages>13</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Mycological research, 2007-04, Vol.111 (4), p.443-455 |
| issn | 0953-7562 1469-8102 |
| language | eng |
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| source | ScienceDirect Journals |
| subjects | Agaricales - chemistry Agaricales - genetics Amino Acid Sequence Basidiomycetes Basidiomycota Basidiomycota - chemistry Basidiomycota - genetics Basidiomycota - metabolism Cacao Cacao - virology ethylene Ethylenes - biosynthesis fungal proteins Fungal Proteins - genetics Fungal Proteins - metabolism gene expression regulation host plants Molecular Sequence Data Moniliophthora Moniliophthora perniciosa Mycelium - metabolism Necrosis Plant Diseases - microbiology Plant Leaves - metabolism plant pathogenic fungi Plant pathology recombinant fusion proteins Sequence Alignment sequence analysis Theobroma cacao WBD witches' broom |
| title | Characterization of necrosis and ethylene-inducing proteins (NEP) in the basidiomycete Moniliophthora perniciosa, the causal agent of witches' broom in Theobroma cacao |
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