Identification of Protein Components in in vivo Human Acquired Enamel Pellicle Using LC−ESI−MS/MS

The acquired enamel pellicle is a thin protein film forming upon exposure of tooth enamel surfaces to saliva. The structural analysis of this integument relies on efficient pellicle harvesting and protein identification procedures. Material from three individual subjects and two pooled samples yield...

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Bibliographic Details
Published in:Journal of proteome research 2007-06, Vol.6 (6), p.2152-2160
Main Authors: Siqueira, Walter L, Zhang, Weimin, Helmerhorst, Eva J, Gygi, Steven P, Oppenheim, Frank G
Format: Article
Language:English
Subjects:
Adult
Amino Acid Sequence
Chromatography, High Pressure Liquid - methods
Dental Pellicle - chemistry
Female
Humans
Male
Molecular Sequence Data
Nanotechnology - methods
Peptides - analysis
Proteins - analysis
Proteomics - methods
Spectrometry, Mass, Electrospray Ionization - methods
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Summary:The acquired enamel pellicle is a thin protein film forming upon exposure of tooth enamel surfaces to saliva. The structural analysis of this integument relies on efficient pellicle harvesting and protein identification procedures. Material from three individual subjects and two pooled samples yielded the identification by LC−ESI−MS/MS of 130 pellicle proteins of which 89 were found in three or more experiments. A high intersubject consistency in pellicle composition was observed. Keywords: acquired enamel pellicle • proteomics • oral • mass spectrometry • LC−ESI−MS/MS • linear ion trap • proteins • saliva
ISSN:1535-3893
1535-3907
DOI:10.1021/pr060580k