Measurement of Trace Elements in Proteins Extracted from Liver by Size Exclusion Chromatography-Inductively Coupled Plasma-Mass Spectrometry with a Magnetic Sector Mass Spectrometer

Proteins are extracted from liver into aqueous buffer at pH 7 and separated by size exclusion chromatography (SEC). Inductively coupled plasma mass spectrometry (ICP-MS) with a magnetic sector mass spectrometer is used to identify those protein fractions that contain Cu, Zn, Mn, Fe, Cd, S, P, Mo, Co...

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Bibliographic Details
Published in:Analytical biochemistry 2001-01, Vol.288 (1), p.89-96
Main Authors: Wang, Jin, Dreessen, Dawn, Wiederin, Daniel R., Houk, R.S.
Format: Article
Language:English
Subjects:
Animals
Calibration
Cattle
Chromatography, Gel - methods
Chromatography, High Pressure Liquid - methods
Hydrogen-Ion Concentration
ICP-MS
Liver - chemistry
Magnetics
Mass Spectrometry - methods
metalloproteins
Metallothionein - analysis
Metals - analysis
Molecular Weight
Osmolar Concentration
Proteins - chemistry
size exclusion chromatography
Trace Elements - analysis
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Summary:Proteins are extracted from liver into aqueous buffer at pH 7 and separated by size exclusion chromatography (SEC). Inductively coupled plasma mass spectrometry (ICP-MS) with a magnetic sector mass spectrometer is used to identify those protein fractions that contain Cu, Zn, Mn, Fe, Cd, S, P, Mo, Co, Ca, or Mg. The experimental setup provides very high sensitivity. Measurements at medium spectral resolution remove polyatomic interferences for some difficult elements like Fe, S, and P. Some elements are found in different molecular weight proteins; for example, cadmium binds to four different protein fractions (>400 kDa, 70 kDa, and metallothionein). Other elements like Mo, Ca, and Mg are present only in low-molecular-weight proteins or other small molecules.
ISSN:0003-2697
1096-0309
DOI:10.1006/abio.2000.4885