ND4 mutation in transitional cell carcinoma: Does mitochondrial mutation occur before tumorigenesis?

To investigate how mitochondrial mutation occurs in cancers, we analyzed ND4 mutation in 53 transitional cell carcinomas (TCCs) of the upper urinary tract and the normal counterpart (perirenal soft tissue). Three methods, i.e., DNA sequencing, restriction fragment length polymorphism (RFLP), and den...

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Bibliographic Details
Published in:Mitochondrion 2007-07, Vol.7 (4), p.273-278
Main Authors: Tzen, Chin-Yuan, Mau, Bey-Liing, Wu, Tsu-Yen
Format: Article
Language:English
Subjects:
Aged
Aged, 80 and over
Amino Acids - genetics
Base Sequence
Carcinoma, Transitional Cell - classification
Carcinoma, Transitional Cell - genetics
Carcinoma, Transitional Cell - pathology
Chromatography, Liquid
Female
Humans
Male
Middle Aged
Mitochondria - genetics
Mutation - genetics
NADH Dehydrogenase - genetics
Nucleotides - genetics
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Summary:To investigate how mitochondrial mutation occurs in cancers, we analyzed ND4 mutation in 53 transitional cell carcinomas (TCCs) of the upper urinary tract and the normal counterpart (perirenal soft tissue). Three methods, i.e., DNA sequencing, restriction fragment length polymorphism (RFLP), and denaturing high-performance liquid chromatography (DHPLC), were employed because of their different sensitive of detecting mutation. The results of sequencing and RFLP showed that ND4 mutations were only found in 24.5% (13/53) of tumor. However, 11 of these mutations could also be identified in the normal tissue by DHPLC, indicating that most mitochondrial mutations identified in tumors preexist as minor components, which are too low in quantity to be detected by less sensitive methods such as DNA sequencing. The result suggests that mtDNA mutation occurs before tumorigenesis and become apparent in cancer cells.
ISSN:1567-7249
DOI:10.1016/j.mito.2007.04.004