Two Aspergillus nidulans genes encoding methylenetetrahydrofolate reductases are up-regulated by homocysteine

Methylenetetrahydrofolate reductase (MTHFR) catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a co-substrate in the synthesis of methionine from homocysteine. We have cloned and characterized two Aspergillus nidulans genes encoding MTHFRs: metA and metF. Mutation...

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Bibliographic Details
Published in:Fungal genetics and biology 2007-07, Vol.44 (7), p.691-700
Main Authors: Sieńko, Marzena, Natorff, Renata, Zieliński, Zbigniew, Hejduk, Arkadiusz, Paszewski, Andrzej
Format: Article
Language:English
Subjects:
Aspergillus nidulans
Aspergillus nidulans - genetics
Aspergillus nidulellus
enzyme activity
gene expression regulation
Gene Expression Regulation, Fungal
gene induction
genes
Genes, Fungal
Homocysteine
metA gene
metF gene
methylenetetrahydrofolate reductase
Methylenetetrahydrofolate Reductase (NADPH2) - genetics
Methylenetetrahydrofolate reductase genes
Molecular Sequence Data
mutants
mutation
nucleotide sequences
phenotype
Regulation by homocysteine
Up-Regulation
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Summary:Methylenetetrahydrofolate reductase (MTHFR) catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a co-substrate in the synthesis of methionine from homocysteine. We have cloned and characterized two Aspergillus nidulans genes encoding MTHFRs: metA and metF. Mutations in either gene result in methionine requirement; the metA-encoded enzyme is responsible for only 10–15% of total MTHFR activity. These two enzymes belong to different classes of MTHFRs. Mutations in metA but not in the metF gene are suppressed by mutations resulting in enhancement of homocysteine synthesis. The expression of both genes is up-regulated by homocysteine.
ISSN:1087-1845
1096-0937
DOI:10.1016/j.fgb.2006.12.002