Electrochromatography in poly(dimethyl)siloxane microchips using organic monolithic stationary phases

This paper shows the in situ synthesis of an hexyl acrylate monolith in PDMS microfluidic devices and its subsequent use as stationary phase for electrochromatography on chip. To overcome the ability of PDMS material to absorb organic monomers, surface modification of the enclosed channels was reali...

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Bibliographic Details
Published in:Electrophoresis 2007-06, Vol.28 (11), p.1668-1673
Main Authors: Faure, Karine, Blas, Maximilien, Yassine, Omar, Delaunay, Nathalie, Crétier, Gérard, Albert, Monique, Rocca, Jean-Louis
Format: Article
Language:English
Subjects:
Acrylate monolith
Capillary Electrochromatography - instrumentation
Capillary Electrochromatography - methods
Dimethylpolysiloxanes - chemistry
Dopamine - isolation & purification
Electrochromatography
Norepinephrine - isolation & purification
PDMS microchip
Silicones - chemistry
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Summary:This paper shows the in situ synthesis of an hexyl acrylate monolith in PDMS microfluidic devices and its subsequent use as stationary phase for electrochromatography on chip. To overcome the ability of PDMS material to absorb organic monomers, surface modification of the enclosed channels was realized by UV‐mediated graft polymerization. This grafting procedure is based on the preliminary adsorption of a photoinitiator onto the PDMS surface and polymerization of charged monomers. Next, hexyl acrylate monoliths were cast in situ using photopolymerization process. The chromatographic behavior of the monolithic column was confirmed by the successful separation of derivatized catecholamines in the PDMS device using a 30 mm effective separation length (100 μm×100 μm section). Efficiencies reached up to 200 000 plates per meter.
ISSN:0173-0835
1522-2683
DOI:10.1002/elps.200600566