Quantitative analysis of gene expressions related to inflammation in canine spastic artery after subarachnoid hemorrhage

• Published in: Stroke (1970) 2001-01, Vol.32 (1), p.212-217
• Main Authors: Aihara, Y, Kasuya, H, Onda, H, Hori, T, Takeda, J
• Format: Article
• Language: English
• Subjects: Animals; Basilar Artery - immunology; Basilar Artery - metabolism; Basilar Artery - pathology; Cell Adhesion Molecules - genetics; Cell Adhesion Molecules - metabolism; Chemokines - genetics; Chemokines - metabolism; Collagen - genetics; Collagen - metabolism; Cytokines - genetics; Cytokines - metabolism; Disease Models, Animal; Dogs; Extracellular Matrix - genetics; Extracellular Matrix - metabolism; Fibroblast Growth Factor 2 - genetics; Fibroblast Growth Factor 2 - metabolism; Gene Expression Profiling; Inflammation - genetics; Inflammation - immunology; Inflammation - metabolism; Reverse Transcriptase Polymerase Chain Reaction; Subarachnoid Hemorrhage - complications; Transforming Growth Factor beta - genetics; Transforming Growth Factor beta - metabolism; Vasospasm, Intracranial - genetics; Vasospasm, Intracranial - immunology; Vasospasm, Intracranial - metabolism
• ISSN: 0039-2499; 1524-4628
• DOI: 10.1161/01.str.32.1.212

The possible role of inflammatory reaction of the cerebral artery in the pathogenesis of cerebral vasospasm has been noted in recent studies. We quantitatively measured the levels of expression of genes related to inflammation in the spastic artery in a canine double-hemorrhage model. Twenty dogs were assigned to 4 groups: group D0, control; group D2, dogs killed 2 days after cisternal injection of blood; group D7, dogs given double cisternal injections of blood and killed 7 days after the first injection; and group D14. Angiography was performed twice: on the first day and before the animals were killed. Total RNA was extracted from the basilar artery. The expressions of interleukin (IL)-1alpha, IL-6, IL-8, IL-10, tumor necrosis factor-alpha, E-secretin, fibronectin, intercellular adhesion molecule (ICAM)-1, vascular cell adhesion molecule-1, transforming growth factor-ss, basic fibroblast growth factor, and collagen types I, III, and IV were examined with TaqMan real-time quantitative reverse transcription-polymerase chain reaction. Prolonged arterial narrowing peaking on 7 day was observed. There was a significant difference in vessel caliber between D0, D2, D7, and D14 groups (P: