Polymorphisms Of The Renin Gene Promoter In Spontaneously Hypertensive And Wistar-Kyoto Rats

SUMMARY 1. In the present study, 1.39 kb of the renin gene 5′ region in spontaneously hypertensive rats (SHR) and Wistar‐Kyoto (WKY) rats was amplified by polymerase chain reaction from genomic DNA and sequenced. Consistent differences in the renin gene sequence of SHR and WKY rats were found at pos...

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Bibliographic Details
Published in:Clinical and experimental pharmacology & physiology 2001-01, Vol.28 (1-2), p.60-63
Main Author: Leckie, Brenda J
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Molecular Sequence Data
Polymorphism, Genetic - genetics
Promoter Regions, Genetic - genetics
Rats
Rats, Inbred SHR - genetics
Rats, Inbred WKY - genetics
Renin - genetics
renin gene polymorphism
renin gene promoter
spontaneously hypertensive rats
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Summary:SUMMARY 1. In the present study, 1.39 kb of the renin gene 5′ region in spontaneously hypertensive rats (SHR) and Wistar‐Kyoto (WKY) rats was amplified by polymerase chain reaction from genomic DNA and sequenced. Consistent differences in the renin gene sequence of SHR and WKY rats were found at positions –725, –727, –979 and –1126/–1129 as numbered from the transcription start site (+1). No polymorphism was specific to hypertensive rats. 2. Gel‐shift assays were performed using labelled SHR renin promotor DNA and nuclear proteins extracted from rat kidneys. The regions between –1122 and –1139 and between –701 and –797 showed protein binding.
ISSN:0305-1870
1440-1681
DOI:10.1046/j.1440-1681.2001.03397.x