The effect of superoxide dismutase on nitric oxide‐mediated and electrical field‐stimulated diabetic rabbit cavernosal smooth muscle relaxation

• Published in: BJU international 2001-01, Vol.87 (1), p.98-103
• Main Authors: Khan, M.A., Thompson, C.S., Jeremy, J.Y., Mumtaz, F.H., Mikhailidis, P., Morgan, R.J.
• Format: Article
• Language: English
• Subjects: Alloxan; Animals; Biological and medical sciences; diabetes mellitus; Diabetes Mellitus, Experimental - enzymology; Diabetes Mellitus, Experimental - physiopathology; Electric Stimulation; erectile dysfunction; Erectile Dysfunction - enzymology; Genital system. Reproduction; Male; Medical sciences; Muscle Relaxation - physiology; Muscle, Smooth - enzymology; Nitric Oxide - physiology; Penis - enzymology; Penis - physiology; Pharmacology. Drug treatments; rabbit; Rabbits; superoxide dismutase; Superoxide Dismutase - pharmacology
• ISSN: 1464-4096; 1464-410X
• DOI: 10.1046/j.1464-410x.2001.00965.x

Objective To investigate the effect of superoxide dismutase (SOD, the enzyme that accelerates the breakdown of the superoxide anion, O2– to H2O) on nitric oxide (NO)‐mediated and electrical field stimulated (EFS) relaxation in diabetic rabbit cavernosal smooth muscle. Materials and methods Diabetes was induced with alloxan (65 mg/kg) in six adult New Zealand White rabbits. After 6 months, cavernosal smooth muscle strips from age‐matched controls and diabetic animals were mounted in organ baths. After precontraction with phenylephrine (10 µmol/L) in the presence of atropine (1 µmol/L), guanethidine (5 µmol/L) and indomethacin (10 µmol/L), relaxation responses to EFS (1–20 Hz), carbachol (10−8−10−4 mol/L) and sodium nitroprusside (SNP, 10−9−10−4 mol/L) were assessed in the presence and absence of SOD (100 IU/mL). Results SNP‐ and carbachol‐mediated (endothelium‐independent and ‐dependent, respectively) relaxations were impaired in the diabetic cavernosal smooth muscle strips compared with controls (concentration required for 50% inhibition, 1.4 µmol/L for diabetic and 0.75 µmol/L for control with SNP, and 44 µmol/L for diabetic and 0.4 µmol/L for control with carbachol). SOD significantly enhanced both SNP‐ and carbachol‐mediated diabetic cavernosal smooth muscle relaxations (both P