Chicken Breast Muscle Connectin: Passive Tension and I-Band Region Primary Structure

We performed cDNA cloning of chicken breast muscle connectin. Together with previous results, our analysis elucidated a 24.2 kb sequence encoding the amino terminus of the protein. This corresponded to the I-band region of the skeletal muscle sarcomere, which is involved in extension and contraction...

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Bibliographic Details
Published in:Journal of molecular biology 2007-07, Vol.370 (2), p.213-219
Main Authors: Noguchi, Hiroshi, Takemori, Shigeru, Kajiwara, Junpei, Kimura, Masako, Maruyama, Koscak, Kimura, Sumiko
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Chickens
Connectin
Humans
Molecular Sequence Data
Muscle Proteins - chemistry
Muscle Proteins - physiology
Muscle Tonus
Muscle, Skeletal - chemistry
Muscle, Skeletal - physiology
Muscles - chemistry
Muscles - physiology
passive tension
PEVK
Protein Kinases - chemistry
Protein Kinases - physiology
Rabbits
Sarcomeres - chemistry
Sarcomeres - physiology
Sequence Alignment
Sequence Homology
striated muscle
titin
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Summary:We performed cDNA cloning of chicken breast muscle connectin. Together with previous results, our analysis elucidated a 24.2 kb sequence encoding the amino terminus of the protein. This corresponded to the I-band region of the skeletal muscle sarcomere, which is involved in extension and contraction between the Z-line and the A–I junction. There were fewer middle immunoglobulin domains and amino acid residues in the PEVK segment of chicken breast muscle connectin than in human skeletal muscle connectin, but more than in human cardiac muscle connectin. We measured passive tension generation by stretching mechanically skinned myofibril bundles. This revealed that appreciable tension development in chicken breast muscle began at longer sarcomere spacings than in rabbit cardiac muscle, but at shorter spacings than in rabbit psoas and soleus muscles. We suggest that the chicken breast muscle sarcomere remains in a relatively extended state even in unstrained sarcomeres. This would explain why chicken breast muscle does not extend under force to the same degree as rabbit psoas and soleus muscles.
ISSN:0022-2836
1089-8638
DOI:10.1016/j.jmb.2007.04.054