Simultaneous determination of cefotaxime and desacetylcefotaxime in human plasma and cerebrospinal fluid by high-performance liquid chromatography

A simple and sensitive HPLC method for the simultaneous determination of cefotaxime (I) and desacetylcefotaxime (II) in human plasma and cerebrospinal fluid (CSF) is described. The assay involves deproteinisation and subsequent separation on a reversed-phase HPLC column, with ultraviolet detection a...

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Bibliographic Details
Published in:Journal of chromatography. B, Biomedical sciences and applications Biomedical sciences and applications, 2001-01, Vol.750 (1), p.171-176
Main Authors: Scanes, T, Hundt, A.F, Swart, K.J, Hundt, H.K.L
Format: Article
Language:English
Subjects:
Analysis
Biological and medical sciences
Cefotaxime
Cefotaxime - analogs & derivatives
Cefotaxime - blood
Cefotaxime - cerebrospinal fluid
Cephalosporins - blood
Cephalosporins - cerebrospinal fluid
Chromatography, High Pressure Liquid - methods
Desacetylcefotaxime
General pharmacology
Humans
Medical sciences
Pharmacology. Drug treatments
Reproducibility of Results
Sensitivity and Specificity
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Summary:A simple and sensitive HPLC method for the simultaneous determination of cefotaxime (I) and desacetylcefotaxime (II) in human plasma and cerebrospinal fluid (CSF) is described. The assay involves deproteinisation and subsequent separation on a reversed-phase HPLC column, with ultraviolet detection at 262 nm. Retention times were 6.8 and 2.2 min for cefotaxime and desacetylcefotaxime, respectively. Average recoveries for the analytes were 78% (I) and 88% (II) from both matrices. Linear responses were observed over a wide range (0.58–940 μg/ml for (I) in plasma, 0.80–55.8 μg/ml for (I) in CSF, 0.54–148 μg/ml for (II) in plasma and 0.50–36.0 μg/ml for (II) in CSF).
ISSN:0378-4347
1387-2273
DOI:10.1016/S0378-4347(00)00417-5