Membrane ion-exchange chromatography for process-scale antibody purification

The large-scale production of recombinant monoclonal antibodies demands economical purification processes with high throughputs. The potential for ion-exchange membrane adsorbers to replace traditional ion-exchange columns was evaluated. Breakthrough capacities of commercially available cation-excha...

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Bibliographic Details
Published in:Journal of Chromatography A 2001-01, Vol.907 (1), p.145-154
Main Authors: Knudsen, Heather L, Fahrner, Robert L, Xu, Yuan, Norling, Lenore A, Blank, Gregory S
Format: Article
Language:English
Subjects:
Antibodies
Antibodies - isolation & purification
Biological and medical sciences
Biotechnology
Chromatography, Ion Exchange - methods
Fundamental and applied biological sciences. Psychology
Health. Pharmaceutical industry
Industrial applications and implications. Economical aspects
Membranes, Artificial
Monoclonal antibodies
Production of active biomolecules
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Summary:The large-scale production of recombinant monoclonal antibodies demands economical purification processes with high throughputs. The potential for ion-exchange membrane adsorbers to replace traditional ion-exchange columns was evaluated. Breakthrough capacities of commercially available cation-exchange membranes were determined as a function of flow-rate and layer number. Due to economic and process restrictions, cation-exchange membranes may not currently be advantageous for process-scale antibody purification in a bind and elute mode. However, anion-exchange membranes in a flow-through mode may provide a reasonable alternative to columns for the removal of low levels of impurities such as DNA, host cell protein, and virus.
ISSN:0021-9673
DOI:10.1016/S0021-9673(00)01041-4