In vitro comparison of the biological effects of three transfection methods for magnetically labeling mouse embryonic stem cells with ferumoxides

In vivo MRI of stem cells (SCs) is an emerging application to evaluate the role of cell therapy in restoring the injured myocardium. The high spatial and temporal resolution combined with iron‐oxide‐based intracellular labeling techniques will provide a sensitive, noninvasive, dual imaging modality...

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Bibliographic Details
Published in:Magnetic resonance in medicine 2007-06, Vol.57 (6), p.1173-1179
Main Authors: Suzuki, Yoriyasu, Zhang, Sally, Kundu, Pratima, Yeung, Alan C., Robbins, Robert C., Yang, Phillip C.
Format: Article
Language:English
Subjects:
Alginates
Analysis of Variance
Animals
Apoptosis
cellular MRI
Contrast Media - pharmacokinetics
Dextrans
Electroporation
Embryonic Stem Cells - metabolism
Embryonic Stem Cells - physiology
Ferrosoferric Oxide
ferum oxides
In Vitro Techniques
Iron - pharmacokinetics
Magnetic Resonance Imaging
Magnetite Nanoparticles
Mice
mouse embryonic stem cells
Oxides - pharmacokinetics
Polylysine - analogs & derivatives
Protamines
Reactive Oxygen Species - metabolism
Reverse Transcriptase Polymerase Chain Reaction
stem cell biology
superparamagnetic iron oxide agents (SPIO)
Transfection - methods
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Summary:In vivo MRI of stem cells (SCs) is an emerging application to evaluate the role of cell therapy in restoring the injured myocardium. The high spatial and temporal resolution combined with iron‐oxide‐based intracellular labeling techniques will provide a sensitive, noninvasive, dual imaging modality for both cells and myocardium. In order to facilitate this novel imaging approach, much effort has been directed towards developing efficient transfection methods. While techniques utilizing poly‐L‐lysine (PLL), protamine sulfate (PS), and electroporation (ELP) have been proposed, the fundamental biological effects of these methods on mouse embryonic SCs (mESC) have not been investigated systematically. In this study a longitudinal in vitro evaluation of cellular viability, apoptosis, proliferation, and cardiac differentiation of magnetically labeled mESC was conducted. No significant difference was seen in these biological parameters among the three transfection methods. However, cardiac differentiation was most attenuated by ELP, and iron uptake was most effective by PS. Magn Reson Med 57:1173–1179, 2007. © 2007 Wiley‐Liss, Inc.
ISSN:0740-3194
1522-2594
DOI:10.1002/mrm.21219