Optoelectric biosensor using indium-tin-oxide electrodes

The use of an optically thin indium-tin-oxide (ITO) electrode is presented for an optoelectric biosensor simultaneously recording optical images and microimpedance to examine time-dependent cellular growth. The transmittance of a 100 nm thick ITO electrode layer is approximately the same as the tran...

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Bibliographic Details
Published in:Optics letters 2007-06, Vol.32 (11), p.1405-1407
Main Authors: CHANG KYOUNG CHOI, KIHM, Kenneth D, ENGLISH, Anthony E
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Biosensing Techniques
Biosensors
Biotechnology
Cell Adhesion
Electrochemistry - methods
Electrodes
Equipment Design
Fundamental and applied biological sciences. Psychology
Indium - chemistry
Methods. Procedures. Technologies
Models, Statistical
Nanoparticles
Optics and Photonics
Silicon - chemistry
Tin Compounds - chemistry
Various methods and equipments
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Summary:The use of an optically thin indium-tin-oxide (ITO) electrode is presented for an optoelectric biosensor simultaneously recording optical images and microimpedance to examine time-dependent cellular growth. The transmittance of a 100 nm thick ITO electrode layer is approximately the same as the transmittance of a clean glass substrate, whereas the industry-standard Au(47.5 nm)/Ti(2.5 nm) electrode layer drops the transmittance to less than 10% of that of the glass substrate. The simultaneous optoelectric measurements permit determining the correlation of the cell-covered area increase with the microimpedance increase, and the example results obtained for live porcine pulmonary artery endothelial cells delineate the quantitative and comprehensive nature of cellular attachment and spreading to the substrate, which has not been clearly perceived before.
ISSN:0146-9592
1539-4794
DOI:10.1364/OL.32.001405