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Overexpression of the G-protein inwardly rectifying potassium channel 1 (GIRK1) in primary breast carcinomas correlates with axillary lymph node metastasis

The acquisition of genetic alterations in tumor cells is a hallmark of cancer progression. Genetic alterations, including chromosomal sequence alterations and abnormal gene expression, increase the malignant potential of tumors by affecting pathways that regulate cell growth, cell death, tumor angio...

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Published in:Cancer research (Chicago, Ill.) Ill.), 2001-01, Vol.61 (2), p.582-588
Main Authors: STRINGER, Bradley K, COOPER, Amiel G, SHEPARD, Scott B
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COOPER, Amiel G
SHEPARD, Scott B
description The acquisition of genetic alterations in tumor cells is a hallmark of cancer progression. Genetic alterations, including chromosomal sequence alterations and abnormal gene expression, increase the malignant potential of tumors by affecting pathways that regulate cell growth, cell death, tumor angiogenesis, and invasion/metastasis. We used an expression profiling technique, representational difference analysis, to identify genes the expressions of which are aberrantly increased in invasive breast carcinomas as compared with adjacent normal breast tissue from the same individual. Among the genes we identified was GIRK1, which encodes a 501 amino acid, G-protein inwardly rectifying potassium channel protein. We then measured GIRK1 mRNA expression in benign breast tissues, primary invasive breast carcinomas, and metastatic breast carcinomas from axillary lymph nodes using quantitative TaqMan reverse transcription-PCR and correlated the results with clinical parameters. We found that GIRK1 overexpression correlated with lymph node metastasis (P < 0.0029), and overexpression was greatest in tumors with more than one positive lymph node. These results indicate that GIRK1 may be useful as a biomarker for lymph node metastasis and possibly a pharmaceutical target.
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We found that GIRK1 overexpression correlated with lymph node metastasis (P &lt; 0.0029), and overexpression was greatest in tumors with more than one positive lymph node. These results indicate that GIRK1 may be useful as a biomarker for lymph node metastasis and possibly a pharmaceutical target.</abstract><cop>Philadelphia, PA</cop><pub>American Association for Cancer Research</pub><pmid>11212253</pmid><tpages>7</tpages></addata></record>
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source EZB Free E-Journals
subjects Adult
Aged
Aged, 80 and over
Base Sequence
Biological and medical sciences
Breast - metabolism
Breast - pathology
Breast Neoplasms - genetics
Breast Neoplasms - pathology
DNA, Complementary - chemistry
DNA, Complementary - genetics
Expressed Sequence Tags
Female
G Protein-Coupled Inwardly-Rectifying Potassium Channels
Gene Expression Regulation, Neoplastic
Gynecology. Andrology. Obstetrics
Humans
Lymph Nodes - metabolism
Lymph Nodes - pathology
Lymphatic Metastasis
Mammary gland diseases
Medical sciences
Middle Aged
Molecular Sequence Data
Neoplasm Invasiveness - genetics
Neoplasm Invasiveness - pathology
Polymerase Chain Reaction - methods
Potassium Channels - genetics
Potassium Channels, Inwardly Rectifying
Reverse Transcriptase Polymerase Chain Reaction
RNA - genetics
RNA - metabolism
RNA, Messenger - genetics
RNA, Messenger - metabolism
Sequence Analysis, DNA
Tumors
title Overexpression of the G-protein inwardly rectifying potassium channel 1 (GIRK1) in primary breast carcinomas correlates with axillary lymph node metastasis
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