Detection of a mixed-backbone oligonucleotide (GEM 231) in liver and tumor tissues by capillary electrophoresis

• Published in: Journal of chromatography. B, Biomedical sciences and applications Biomedical sciences and applications, 2001-01, Vol.750 (1), p.129-135
• Main Authors: Bansal, Reeta, Chen, Helen X, Marshall, John L, Tan, Jie, Glazer, Robert I, Wainer, Irving W
• Format: Article
• Language: English
• Subjects: Analysis; Animals; Biological and medical sciences; Electrophoresis, Capillary - methods; GEM 231; General pharmacology; Humans; Liver - chemistry; Medical sciences; Mice; Mice, Nude; Neoplasm Transplantation; Neoplasms - chemistry; Oligonucleotides; Oligonucleotides - analysis; Pharmacology. Drug treatments; Reproducibility of Results; Sensitivity and Specificity
• ISSN: 0378-4347; 1387-2273
• DOI: 10.1016/S0378-4347(00)00436-9

A simple, rapid and sensitive method has been developed and validated for the analysis of a mixed-backbone oligonucleotide (GEM 231) in tumor tissues. The analysis was performed using a capillary electrophoresis (CE) system with UV detection. An extended light path (bubble cell) capillary column of 64.5 cm (effective length 56 cm)×50 μm I.D. is used as the separation column. The optimized chromatographic conditions were background electrolyte: sodium borate buffer (60 m M, pH 9.1), electrokinetic injection: 10 s, applied voltage: 30 kV, detection at λ=210 nm. A linear relationship was observed between the peak area and the amount of GEM 231 in the range of 1.0–1000 μg/ml. The lower detection limit of the drug was 100 pg with an average recovery of about 75±5%. The inter-day and intra-day relative standard deviations were