Investigation of avenacin-deficient mutants of Avena strigosa

The biosynthesis of cyclic triterpenoids in ten saponin-deficient ( sad) mutant varieties of the diploid oat Avena strigosa is reported. Two mutants were found to be deficient in 2,3-oxidosqualene: β-amyrin cyclase (OS βAC) (EC 5.4.99) and thus unable to produce the β-amyrin necessary for the produc...

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Bibliographic Details
Published in:Phytochemistry (Oxford) 2001-01, Vol.56 (2), p.121-129
Main Authors: Trojanowska, Miranda R, Osbourn, Anne E, Daniels, Mike J, Threlfall, David R
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Avena - chemistry
Avena - enzymology
Avena - genetics
Avena strigosa
Avenacins
Biological and medical sciences
Biosynthesis
Carbon Radioisotopes
Chromatography, High Pressure Liquid
Economic plant physiology
Fundamental and applied biological sciences. Psychology
Intramolecular Transferases - metabolism
Metabolism
Metabolism. Physicochemical requirements
Mevalonic Acid - chemistry
Mutants
Mutation
Nitrogen metabolism and other ones (excepting carbon metabolism)
Nutrition. Photosynthesis. Respiration. Metabolism
Phytosterols
Plant physiology and development
Poaceae
Saponins
Saponins - genetics
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Summary:The biosynthesis of cyclic triterpenoids in ten saponin-deficient ( sad) mutant varieties of the diploid oat Avena strigosa is reported. Two mutants were found to be deficient in 2,3-oxidosqualene: β-amyrin cyclase (OS βAC) (EC 5.4.99) and thus unable to produce the β-amyrin necessary for the production of avenacins. The other mutants studied had post β-amyrin lesions. 2,3-Oxidosqualene:cycloartenol cyclase (OSCC) (EC 5.4.99.8) needed for sterol formation was present in all ten mutants.
ISSN:0031-9422
1873-3700
DOI:10.1016/S0031-9422(00)00399-X