Effects of collagen IV and laminin on the reconstruction of human oral mucosa

• Published in: Journal of biomedical materials research 2001, Vol.58 (1), p.108-112
• Main Authors: Kim, Suk W., Park, Kyoung C., Kim, Hye J., Cho, Kwang H., Chung, Jin H., Kim, Kyu H., Eun, Hee C., Lee, Jae S., Park, Kee D.
• Format: Article
• Language: English
• Subjects: Animals; basement membrane; Basement Membrane - chemistry; Biological and medical sciences; Biomarkers; Cell Differentiation - drug effects; Cells, Cultured - drug effects; Child; Child, Preschool; Collagen - pharmacology; Culture Techniques - instrumentation; Culture Techniques - methods; Epithelial Cells - chemistry; Epithelial Cells - cytology; Epithelial Cells - drug effects; Gels; Head and neck surgery. Maxillofacial surgery. Dental surgery. Orthodontics; Humans; Intermediate Filament Proteins - analysis; Keratinocytes - chemistry; Keratinocytes - cytology; Keratinocytes - drug effects; Keratins - analysis; laminin; Laminin - pharmacology; Medical sciences; Microscopy, Electron; Mouth Mucosa - cytology; Mouth Mucosa - drug effects; mucosa equivalent; Protein Isoforms - analysis; Protein Precursors - analysis; Rats; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Surgery of the upper aerodigestive tract; Tail - chemistry; type IV collagen
• ISSN: 0021-9304; 1097-4636
• DOI: 10.1002/1097-4636(2001)58:1<108::AID-JBM160>3.0.CO;2-I

To investigate the effects of basement membrane proteins on the reconstruction of mucosa equivalent, oral mucosa substitute were cultured on (1) type I collagen gels, (2) type IV collagen‐coated type I collagen gels, (3) laminin‐coated type I collagen gels, and (4) type I collagen gels containing both type IV collagen and laminin. H/E and PAS staining showed that the characteristics of the oral mucosa were preserved under all the experimental conditions. However, the basal keratinocytes appeared cuboidal when the type I collagen gels were coated with type IV collagen plus laminin. The expression of the differentiation markers was similar, but weak staining of filaggrin, K13, and involucrin was observed with the type IV collagen plus laminin coating. Furthermore, electron microscopy revealed that the size of the basal keratinocytes was relatively small and uniform when both type IV collagen and laminin were used. These findings suggested that these two major basement membrane proteins are important in the process of differentiation in mucosal keratinocytes. © 2000 John Wiley & Sons, Inc. J Biomed Mater Res (Appl Biomater) 58: 108–112, 2001