Binding of Nicotinic Ligands to and Nicotine-Induced Calcium Signaling in Trypanosoma cruzi

This work provides evidence that nicotine (1 × 10−5 M) can cause changes in the intracellular calcium concentration of Trypanosoma cruzi epimastigotes, which can be blocked by α-bungarotoxin but not by atropine. Moreover, parasite membranes also bind such nicotinic acetylcholine receptor antagonist...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2001-02, Vol.281 (2), p.300-304
Main Authors: Bollo, Mariana, Venera, Graciela, de Jiménez Bonino, Mirtha Biscoglio, Machado-Domenech, Estela
Format: Article
Language:English
Subjects:
Animals
Binding, Competitive - drug effects
Bungarotoxins - metabolism
calcium
Calcium - metabolism
Calcium Signaling
Carbachol - metabolism
Carbachol - pharmacology
carbamylcholine
Cholinergic Agonists - metabolism
Dose-Response Relationship, Drug
Fura-2
Membranes - drug effects
Membranes - metabolism
nicotine
Nicotine - metabolism
Nicotine - pharmacology
nicotinic ligands
Trypanosoma cruzi
Trypanosoma cruzi - drug effects
Trypanosoma cruzi - metabolism
α-bungarotoxin
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Summary:This work provides evidence that nicotine (1 × 10−5 M) can cause changes in the intracellular calcium concentration of Trypanosoma cruzi epimastigotes, which can be blocked by α-bungarotoxin but not by atropine. Moreover, parasite membranes also bind such nicotinic acetylcholine receptor antagonist as well as agonists such as carbamylcholine (IC50: 7.6 × 10−7 M) and nicotine (IC50: 1 × 10−7 M). Results suggest that there is a molecular species in the surface of the parasite able to bind nicotinic ligands; therefore, nicotine interaction could lead to the activation of the mechanisms involved in intracellular calcium concentration increase in the parasite.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.2001.4344