First derivative spectrophotometric, TLC-densitometric, and HPLC determination of acebutolol HCL in presence of its acid-induced degradation product

Three methods are presented for the determination of acebutolol HCl in presence of its acid-induced degradation product. The first method was based on measurement of the first derivative amplitude of acebutolol HCl at 266.6 nm. The second method was based on separation of acebutolol HCl from its aci...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 2001-02, Vol.24 (4), p.527-534
Main Authors: El-Gindy, Alaa, Ashour, Ahmed, Abdel-Fattah, Laila, Shabana, Marwan M
Format: Article
Language:English
Subjects:
Acebutolol - analysis
Acebutolol HCl
Analysis
Biological and medical sciences
Chromatography, High Pressure Liquid
Chromatography, Thin Layer
Densitometry
First-derivative spectrophotometry
General pharmacology
HPLC
Hydrogen-Ion Concentration
Kinetics
Kinetics of degradation
Medical sciences
Pharmacology. Drug treatments
Spectrophotometry, Infrared
Spectrophotometry, Ultraviolet
Tablets - chemistry
Temperature
TLC-densitometry
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Summary:Three methods are presented for the determination of acebutolol HCl in presence of its acid-induced degradation product. The first method was based on measurement of the first derivative amplitude of acebutolol HCl at 266.6 nm. The second method was based on separation of acebutolol HCl from its acid-induced degradation product followed by densitometric measurement of the spots at 230 nm. The separation was carried out on silica gel 60 F 254, using ethanol–glacial acetic acid (4:1, v/v) as mobile phase. Second order polynomial equation was used for the regression line. The third method was based on high performance liquid chromatographic (HPLC) separation of acebutolol HCl from its acid-induced degradation product on a reversed phase, ODS column using a mobile phase of methanol–water (55:45, v/v) with UV detection at 240 nm. The first derivative spectrophotometric method was utilized to investigate the kinetics of the acid degradation process at different temperatures.
ISSN:0731-7085
1873-264X
DOI:10.1016/S0731-7085(00)00451-9