Structure of the O16 antigen of Stenotrophomonas maltophilia

A polysaccharide containing d-ribose, N-acetyl- d-glucosamine, and N-acetyl- d-mannosamine was isolated from the phenol-soluble lipopolysaccharide extracted from defatted cell walls of the reference strain (560) for serogroup O16 of Stenotrophomonas maltophilia. The results of methylation analysis,...

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Bibliographic Details
Published in:Carbohydrate research 2001-01, Vol.330 (2), p.279-283
Main Authors: Winn, Angela M., Wilkinson, Stephen G.
Format: Article
Language:English
Subjects:
Carbohydrate Conformation
Carbohydrate Sequence
Lipopolysaccharide
Lipopolysaccharides - chemistry
Lipopolysaccharides - isolation & purification
Magnetic Resonance Spectroscopy
Molecular Sequence Data
O Antigens - chemistry
O-Specific polymer
Polysaccharides - chemistry
Polysaccharides - immunology
Polysaccharides - isolation & purification
Stenotrophomonas maltophilia
Stenotrophomonas maltophilia - chemistry
Stenotrophomonas maltophilia - immunology
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Summary:A polysaccharide containing d-ribose, N-acetyl- d-glucosamine, and N-acetyl- d-mannosamine was isolated from the phenol-soluble lipopolysaccharide extracted from defatted cell walls of the reference strain (560) for serogroup O16 of Stenotrophomonas maltophilia. The results of methylation analysis, chemical degradations, and NMR spectroscopy showed that the polysaccharide is based on a branched trisaccharide repeating-unit of the structure shown below. Although ribose was absent from about half of the units in the isolated polymer, the regularity and spacing of the ladder observed on SDS-PAGE of the parent lipopolysaccharide indicate that this was an artefact of the mild acid hydrolysis used to release the polymer. On the other hand, the effects of mild alkaline hydrolysis on the polymer indicated partial O-acetylation.
ISSN:0008-6215
1873-426X
DOI:10.1016/S0008-6215(00)00276-7