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Regulation of inducible adhesion molecule expression in human endothelial cells by grape seed proanthocyanidin extract
Altered expression of cell adhesion molecule expression has been implicated in a variety of chronic inflammatory conditions. Regulation of adhesion molecule expression by specific redox sensitive mechanisms has been reported. Grape seed proanthocyanidins have been reported to have potent antioxidant...
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Published in: | Molecular and cellular biochemistry 2001-01, Vol.216 (1-2), p.1-7 |
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description | Altered expression of cell adhesion molecule expression has been implicated in a variety of chronic inflammatory conditions. Regulation of adhesion molecule expression by specific redox sensitive mechanisms has been reported. Grape seed proanthocyanidins have been reported to have potent antioxidant properties. We evaluated the effects of grape seed proanthocyanidin extract (GSPE) on the expression of TNFalpha-induced ICAM-1 and VCAM-1 expression in primary human umbilical vein endothelial cells (HUVEC). GSPE at low concentrations (1-5 micrograms/ml), down-regulated TNFalpha-induced VCAM-1 expression but not ICAM-1 expression in HUVEC. Such regulation of inducible VCAM-1 by GSPE was also observed at the mRNA expression level. A cell-cell co-culture assay was performed to verify whether the inhibitory effect of GSPE on the expression of VCAM-1 was also effective in down-regulating actual endothelial cell/leukocyte interaction. GSPE treatment significantly decreased TNFalpha-induced adherence of T-cells to HUVEC. Although several studies have postulated NF-kappaB as the molecular site where redox active substances act to regulate agonist-induced ICAM-1 and VCAM-1 gene expression, inhibition of inducible VCAM-1 gene expression by GSPE was not through a NF-kappaB-dependent pathway as detected by a NF-kappaB reporter assay. The potent inhibitory effect of low concentrations of GSPE on agonist-induced VCAM-1 expression suggests therapeutic potential of this extract in inflammatory conditions and other pathologies involving altered expression of VCAM- 1. |
doi_str_mv | 10.1023/A:1011053300727 |
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Regulation of adhesion molecule expression by specific redox sensitive mechanisms has been reported. Grape seed proanthocyanidins have been reported to have potent antioxidant properties. We evaluated the effects of grape seed proanthocyanidin extract (GSPE) on the expression of TNFalpha-induced ICAM-1 and VCAM-1 expression in primary human umbilical vein endothelial cells (HUVEC). GSPE at low concentrations (1-5 micrograms/ml), down-regulated TNFalpha-induced VCAM-1 expression but not ICAM-1 expression in HUVEC. Such regulation of inducible VCAM-1 by GSPE was also observed at the mRNA expression level. A cell-cell co-culture assay was performed to verify whether the inhibitory effect of GSPE on the expression of VCAM-1 was also effective in down-regulating actual endothelial cell/leukocyte interaction. GSPE treatment significantly decreased TNFalpha-induced adherence of T-cells to HUVEC. Although several studies have postulated NF-kappaB as the molecular site where redox active substances act to regulate agonist-induced ICAM-1 and VCAM-1 gene expression, inhibition of inducible VCAM-1 gene expression by GSPE was not through a NF-kappaB-dependent pathway as detected by a NF-kappaB reporter assay. The potent inhibitory effect of low concentrations of GSPE on agonist-induced VCAM-1 expression suggests therapeutic potential of this extract in inflammatory conditions and other pathologies involving altered expression of VCAM- 1.</description><identifier>ISSN: 0300-8177</identifier><identifier>EISSN: 1573-4919</identifier><identifier>DOI: 10.1023/A:1011053300727</identifier><identifier>PMID: 11216853</identifier><language>eng</language><publisher>Netherlands: Springer Nature B.V</publisher><subject>Adhesion ; Anthocyanins - genetics ; Anthocyanins - metabolism ; Blotting, Northern ; Cell Adhesion ; Cell adhesion & migration ; Cells ; Cells, Cultured ; Coculture Techniques ; Dose-Response Relationship, Drug ; Down-Regulation ; Endothelium, Vascular - metabolism ; Flavonoids - metabolism ; Flow Cytometry ; Gene expression ; Gene Expression Regulation ; Genes, Reporter ; Humans ; Intercellular Adhesion Molecule-1 - biosynthesis ; Jurkat Cells ; Leukocytes - metabolism ; Luciferases - metabolism ; Microscopy, Fluorescence ; NF-kappa B - biosynthesis ; Oxidation-Reduction ; Plant Extracts - metabolism ; Proanthocyanidins ; Proteins ; Regulation ; RNA, Messenger - metabolism ; Rosales - metabolism ; T-Lymphocytes - metabolism ; Time Factors ; Transcription, Genetic ; Transcriptional Activation ; Transfection ; Tumor Necrosis Factor-alpha - metabolism ; Umbilical Veins - metabolism ; Vascular Cell Adhesion Molecule-1 - biosynthesis</subject><ispartof>Molecular and cellular biochemistry, 2001-01, Vol.216 (1-2), p.1-7</ispartof><rights>Kluwer Academic Publishers 2001</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c280t-4d6c3824e682b1d27576748f246b6c31c025c80a46bfbbe2c56e99a33a62f8ca3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11216853$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sen, C K</creatorcontrib><creatorcontrib>Bagchi, D</creatorcontrib><title>Regulation of inducible adhesion molecule expression in human endothelial cells by grape seed proanthocyanidin extract</title><title>Molecular and cellular biochemistry</title><addtitle>Mol Cell Biochem</addtitle><description>Altered expression of cell adhesion molecule expression has been implicated in a variety of chronic inflammatory conditions. Regulation of adhesion molecule expression by specific redox sensitive mechanisms has been reported. Grape seed proanthocyanidins have been reported to have potent antioxidant properties. We evaluated the effects of grape seed proanthocyanidin extract (GSPE) on the expression of TNFalpha-induced ICAM-1 and VCAM-1 expression in primary human umbilical vein endothelial cells (HUVEC). GSPE at low concentrations (1-5 micrograms/ml), down-regulated TNFalpha-induced VCAM-1 expression but not ICAM-1 expression in HUVEC. Such regulation of inducible VCAM-1 by GSPE was also observed at the mRNA expression level. A cell-cell co-culture assay was performed to verify whether the inhibitory effect of GSPE on the expression of VCAM-1 was also effective in down-regulating actual endothelial cell/leukocyte interaction. GSPE treatment significantly decreased TNFalpha-induced adherence of T-cells to HUVEC. Although several studies have postulated NF-kappaB as the molecular site where redox active substances act to regulate agonist-induced ICAM-1 and VCAM-1 gene expression, inhibition of inducible VCAM-1 gene expression by GSPE was not through a NF-kappaB-dependent pathway as detected by a NF-kappaB reporter assay. The potent inhibitory effect of low concentrations of GSPE on agonist-induced VCAM-1 expression suggests therapeutic potential of this extract in inflammatory conditions and other pathologies involving altered expression of VCAM- 1.</description><subject>Adhesion</subject><subject>Anthocyanins - genetics</subject><subject>Anthocyanins - metabolism</subject><subject>Blotting, Northern</subject><subject>Cell Adhesion</subject><subject>Cell adhesion & migration</subject><subject>Cells</subject><subject>Cells, Cultured</subject><subject>Coculture Techniques</subject><subject>Dose-Response Relationship, Drug</subject><subject>Down-Regulation</subject><subject>Endothelium, Vascular - metabolism</subject><subject>Flavonoids - metabolism</subject><subject>Flow Cytometry</subject><subject>Gene expression</subject><subject>Gene Expression Regulation</subject><subject>Genes, Reporter</subject><subject>Humans</subject><subject>Intercellular Adhesion Molecule-1 - biosynthesis</subject><subject>Jurkat Cells</subject><subject>Leukocytes - metabolism</subject><subject>Luciferases - metabolism</subject><subject>Microscopy, Fluorescence</subject><subject>NF-kappa B - biosynthesis</subject><subject>Oxidation-Reduction</subject><subject>Plant Extracts - metabolism</subject><subject>Proanthocyanidins</subject><subject>Proteins</subject><subject>Regulation</subject><subject>RNA, Messenger - metabolism</subject><subject>Rosales - metabolism</subject><subject>T-Lymphocytes - metabolism</subject><subject>Time Factors</subject><subject>Transcription, Genetic</subject><subject>Transcriptional Activation</subject><subject>Transfection</subject><subject>Tumor Necrosis Factor-alpha - metabolism</subject><subject>Umbilical Veins - metabolism</subject><subject>Vascular Cell Adhesion Molecule-1 - biosynthesis</subject><issn>0300-8177</issn><issn>1573-4919</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><recordid>eNpdkM1LxDAQxYMo7rp69ibBg7dqJmmT1tuy-AULgui5pMl026VN16aV3f_erK4XYWCY37x5PIaQS2C3wLi4m98DA2CJEIwpro7IFBIlojiD7JhMWaBRCkpNyJn3a8YgFJySCQAHmSZiSr7ecDU2eqg7R7uS1s6Opi4apNpW6Pe07Ro0YyC43fTof1jtaDW22lF0thsqbGrdUINN42mxo6teb5B6REs3fafdUHVmp11twxluh16b4ZyclLrxeHHoM_Lx-PC-eI6Wr08vi_kyMjxlQxRbaUTKY5QpL8BylSip4rTksSzCBgzjiUmZDmNZFMhNIjHLtBBa8jI1WszIza9vCPI5oh_ytvb7oNphN_pcsSTjwTwIr_8J193Yu5AtV4kEBUKxILo6iMaiRZtv-rrV_S7_e6f4BpgPeHA</recordid><startdate>200101</startdate><enddate>200101</enddate><creator>Sen, C K</creator><creator>Bagchi, D</creator><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7QL</scope><scope>7QP</scope><scope>7T5</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200101</creationdate><title>Regulation of inducible adhesion molecule expression in human endothelial cells by grape seed proanthocyanidin extract</title><author>Sen, C K ; Bagchi, D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c280t-4d6c3824e682b1d27576748f246b6c31c025c80a46bfbbe2c56e99a33a62f8ca3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Adhesion</topic><topic>Anthocyanins - genetics</topic><topic>Anthocyanins - metabolism</topic><topic>Blotting, Northern</topic><topic>Cell Adhesion</topic><topic>Cell adhesion & migration</topic><topic>Cells</topic><topic>Cells, Cultured</topic><topic>Coculture Techniques</topic><topic>Dose-Response Relationship, Drug</topic><topic>Down-Regulation</topic><topic>Endothelium, Vascular - metabolism</topic><topic>Flavonoids - metabolism</topic><topic>Flow Cytometry</topic><topic>Gene expression</topic><topic>Gene Expression Regulation</topic><topic>Genes, Reporter</topic><topic>Humans</topic><topic>Intercellular Adhesion Molecule-1 - biosynthesis</topic><topic>Jurkat Cells</topic><topic>Leukocytes - metabolism</topic><topic>Luciferases - metabolism</topic><topic>Microscopy, Fluorescence</topic><topic>NF-kappa B - biosynthesis</topic><topic>Oxidation-Reduction</topic><topic>Plant Extracts - metabolism</topic><topic>Proanthocyanidins</topic><topic>Proteins</topic><topic>Regulation</topic><topic>RNA, Messenger - metabolism</topic><topic>Rosales - metabolism</topic><topic>T-Lymphocytes - metabolism</topic><topic>Time Factors</topic><topic>Transcription, Genetic</topic><topic>Transcriptional Activation</topic><topic>Transfection</topic><topic>Tumor Necrosis Factor-alpha - metabolism</topic><topic>Umbilical Veins - metabolism</topic><topic>Vascular Cell Adhesion Molecule-1 - biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sen, C K</creatorcontrib><creatorcontrib>Bagchi, D</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular and cellular biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sen, C K</au><au>Bagchi, D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regulation of inducible adhesion molecule expression in human endothelial cells by grape seed proanthocyanidin extract</atitle><jtitle>Molecular and cellular biochemistry</jtitle><addtitle>Mol Cell Biochem</addtitle><date>2001-01</date><risdate>2001</risdate><volume>216</volume><issue>1-2</issue><spage>1</spage><epage>7</epage><pages>1-7</pages><issn>0300-8177</issn><eissn>1573-4919</eissn><abstract>Altered expression of cell adhesion molecule expression has been implicated in a variety of chronic inflammatory conditions. Regulation of adhesion molecule expression by specific redox sensitive mechanisms has been reported. Grape seed proanthocyanidins have been reported to have potent antioxidant properties. We evaluated the effects of grape seed proanthocyanidin extract (GSPE) on the expression of TNFalpha-induced ICAM-1 and VCAM-1 expression in primary human umbilical vein endothelial cells (HUVEC). GSPE at low concentrations (1-5 micrograms/ml), down-regulated TNFalpha-induced VCAM-1 expression but not ICAM-1 expression in HUVEC. Such regulation of inducible VCAM-1 by GSPE was also observed at the mRNA expression level. A cell-cell co-culture assay was performed to verify whether the inhibitory effect of GSPE on the expression of VCAM-1 was also effective in down-regulating actual endothelial cell/leukocyte interaction. GSPE treatment significantly decreased TNFalpha-induced adherence of T-cells to HUVEC. Although several studies have postulated NF-kappaB as the molecular site where redox active substances act to regulate agonist-induced ICAM-1 and VCAM-1 gene expression, inhibition of inducible VCAM-1 gene expression by GSPE was not through a NF-kappaB-dependent pathway as detected by a NF-kappaB reporter assay. The potent inhibitory effect of low concentrations of GSPE on agonist-induced VCAM-1 expression suggests therapeutic potential of this extract in inflammatory conditions and other pathologies involving altered expression of VCAM- 1.</abstract><cop>Netherlands</cop><pub>Springer Nature B.V</pub><pmid>11216853</pmid><doi>10.1023/A:1011053300727</doi><tpages>7</tpages></addata></record> |
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subjects | Adhesion Anthocyanins - genetics Anthocyanins - metabolism Blotting, Northern Cell Adhesion Cell adhesion & migration Cells Cells, Cultured Coculture Techniques Dose-Response Relationship, Drug Down-Regulation Endothelium, Vascular - metabolism Flavonoids - metabolism Flow Cytometry Gene expression Gene Expression Regulation Genes, Reporter Humans Intercellular Adhesion Molecule-1 - biosynthesis Jurkat Cells Leukocytes - metabolism Luciferases - metabolism Microscopy, Fluorescence NF-kappa B - biosynthesis Oxidation-Reduction Plant Extracts - metabolism Proanthocyanidins Proteins Regulation RNA, Messenger - metabolism Rosales - metabolism T-Lymphocytes - metabolism Time Factors Transcription, Genetic Transcriptional Activation Transfection Tumor Necrosis Factor-alpha - metabolism Umbilical Veins - metabolism Vascular Cell Adhesion Molecule-1 - biosynthesis |
title | Regulation of inducible adhesion molecule expression in human endothelial cells by grape seed proanthocyanidin extract |
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