Novel method for efficient production of multipotential hematopoietic progenitors from human embryonic stem cells

We propose a novel method for the efficient production of hematopoietic progenitors from human embryonic stem cells (hESC) via coculture with murine fetal liver-derived stromal cells, in which embryonic hematopoiesis dramatically expands at midgestation. We generated various hematopoietic progenitor...

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Bibliographic Details
Published in:International journal of hematology 2007-06, Vol.85 (5), p.371-379
Main Authors: FENG MA, DAN WANG, HANADA, Sachiyo, EBIHARA, Yasuhiro, KAWASAKI, Hirohide, ZAIKE, Yuji, HEIKE, Toshio, NAKAHATA, Tatsutoshi, TSUJI, Kohichiro
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cell Differentiation
Cell Division
Cells, Cultured
Coculture Techniques - methods
Female
Hematologic and hematopoietic diseases
Hematopoiesis, Extramedullary
Hematopoietic Stem Cells - cytology
Humans
Liver - cytology
Liver - embryology
Medical sciences
Mice
Mice, Inbred C57BL
Multipotent Stem Cells - cytology
Pregnancy
Stromal Cells - cytology
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Summary:We propose a novel method for the efficient production of hematopoietic progenitors from human embryonic stem cells (hESC) via coculture with murine fetal liver-derived stromal cells, in which embryonic hematopoiesis dramatically expands at midgestation. We generated various hematopoietic progenitors in coculture, and this hematopoietic activity was concentrated in cobblestone-like cells derived from differentiated hESC. The cobblestone-like cells mostly expressed CD34 and retained an endothelial cell potential. They also contained hematopoietic colony-forming cells, especially erythroid and multilineage colony-forming cells at high frequency. The multipotential hematopoietic progenitors abundant among the cobblestone-like cells produced almost all types of mature blood cells, including adult-type alpha-globin-expressing erythrocytes and tryptase/chymase double-positive mast cells. These progenitors showed neither the immature properties of ESC nor the potential to differentiate into endoderm and ectoderm at a clonal level. The coculture system developed for hESC can provide a novel source of hematopoietic and blood cells for applications in cellular therapy and drug screening.
ISSN:0925-5710
1865-3774
DOI:10.1532/ijh97.06203