Analysis of the antigen recognition sites of anti-methamphetamine monoclonal antibodies (II): Unique feature of MA-3 antibody

A monoclonal antibody against methamphetamine (MA-3 mAb) was found to be strongly bound to ephedrine. This feature was quite different from that of other fourteen mAbs against MA. Analyses of cDNA sequence and steric conformation by molecular modeling revealed that one hydrophilic pocket was generat...

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Bibliographic Details
Published in:Journal of bioscience and bioengineering 2000, Vol.89 (5), p.492-494
Main Authors: Ishimaru, Masanori, Morikawa, Keiko, Hifumi, Emi, Itoh, Takuzi, Uda, Taizo
Format: Article
Language:English
Subjects:
Amino acids
Antigens
Biological and medical sciences
Biotechnology
CDR
DNA sequences
ephedrine
Fundamental and applied biological sciences. Psychology
Health. Pharmaceutical industry
Industrial applications and implications. Economical aspects
Mathematical models
methamphetamine
molecular modeling
Monoclonal antibodies
monoclonal antibody
Organic chemicals
Production of active biomolecules
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Summary:A monoclonal antibody against methamphetamine (MA-3 mAb) was found to be strongly bound to ephedrine. This feature was quite different from that of other fourteen mAbs against MA. Analyses of cDNA sequence and steric conformation by molecular modeling revealed that one hydrophilic pocket was generated in the heavy chain of MA-3 mAb involving CDRH-1 and CDRH-2. Asn33, Asn35, Asn50 and Asp52 were the main components of the unique pocket capable of binding to the hydroxyl group of ephedrine.
ISSN:1389-1723
1347-4421
DOI:10.1016/S1389-1723(00)89103-3