A permease exhibiting a dual role for lysine and biotin uptake in Saccharomyces cerevisiae

Among Saccharomyces cerevisiae strains each defective in one of 11 amino acid permeases, a lysine permease disruptant (DK) exhibited about 2-fold reductions in maximum cell density and fermentation ability compared to the parent in a synthetic medium. These unusual properties of DK were found to res...

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Bibliographic Details
Published in:Journal of bioscience and bioengineering 2000, Vol.89 (1), p.90-93
Main Authors: Kosugi, Akihiko, Koizumi, Yukimichi, Yanagida, Fujiharu, Udaka, Shigezo
Format: Article
Language:English
Subjects:
Amino acids
Biological and medical sciences
biotin transport
biotin uptake
Density (specific gravity)
Fermentation
Fundamental and applied biological sciences. Psychology
gene disruption
Genes
Growth, nutrition, metabolism, transports, enzymes. Molecular biology
lysine permease
lysine transport
Microbiology
Mycology
permease
Saccharomyces cerevisiae
Yeast
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Summary:Among Saccharomyces cerevisiae strains each defective in one of 11 amino acid permeases, a lysine permease disruptant (DK) exhibited about 2-fold reductions in maximum cell density and fermentation ability compared to the parent in a synthetic medium. These unusual properties of DK were found to result from the requirement of biotin for growth, in contrast to the parent whose growth was not dependent on external biotin. The rate of 14C-labeled biotin uptake and the intracellular free biotin content of DK were 2–2.5 fold lower than in the parent. We suggest that lysine permease in S. cerevisiae has the ability to transport both lysine and biotin.
ISSN:1389-1723
1347-4421
DOI:10.1016/S1389-1723(00)88058-5