Rapid detection of homologously integrated DNA fragments and accurate quantitation of their copy number in transgenic Aspergillus oryzae by PCR

A simple and rapid method for the analysis of artificially introduced DNA fragments has been developed using competitive PCR and long and accurate PCR. The locus and the copy number of the DNA fragments in each Aspergillus oryzae transformant could be detected more rapidly and accurately by this met...

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Bibliographic Details
Published in:Journal of bioscience and bioengineering 2000, Vol.90 (5), p.577-579
Main Authors: Honda, Munechika, Minetoki, Toshitaka, Gomi, Katsuya, Machida, Masayuki
Format: Article
Language:English
Subjects:
ASPERGILLUS ORYZAE
Biological and medical sciences
Biotechnology
competitive PCR
DNA
Electrophoresis
Enzymes
Extraction
Fundamental and applied biological sciences. Psychology
gene copy number
Genes
LA-PCR
Methods. Procedures. Technologies
Nitrates
Others
PCR
Spectrophotometers
TRANSGENICS
Various methods and equipments
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Description
Summary:A simple and rapid method for the analysis of artificially introduced DNA fragments has been developed using competitive PCR and long and accurate PCR. The locus and the copy number of the DNA fragments in each Aspergillus oryzae transformant could be detected more rapidly and accurately by this method than by the conventional Southern hybridization method.
ISSN:1389-1723
1347-4421
DOI:10.1016/S1389-1723(01)80046-3