Multilayered DNA coatings: In vitro bioactivity studies and effects on osteoblast-like cell behavior

• Published in: Acta biomaterialia 2007-07, Vol.3 (4), p.587-596
• Main Authors: van den Beucken, J.J.J.P., Walboomers, X.F., Leeuwenburgh, S.C.G., Vos, M.R.J., Sommerdijk, N.A.J.M., Nolte, R.J.M., Jansen, J.A.
• Format: Article
• Language: English
• Subjects: Alkaline Phosphatase - metabolism; Animals; Body Fluids - chemistry; Bone Marrow Cells - cytology; Calcium phosphate; Calcium Phosphates - chemistry; Carbon - chemistry; Cations - chemistry; Cell Differentiation - drug effects; Cell Proliferation - drug effects; Cells, Cultured; Coated Materials, Biocompatible - chemistry; Computer Simulation; DNA - chemistry; DNA - pharmacology; DNA coating; Durapatite - chemistry; Electrolytes - chemistry; Electron Probe Microanalysis; Femur - cytology; In Vitro Techniques; Layer-by-layer deposition; Male; Osteoblast-like cell; Osteoblasts - cytology; Osteoblasts - drug effects; Osteoblasts - physiology; Osteoblasts - ultrastructure; Osteocalcin - analysis; Osteocalcin - metabolism; Polymers - chemistry; Rats; Rats, Wistar; SBF; Static Electricity; Surface Properties; Titanium - chemistry
• ISSN: 1742-7061; 1878-7568
• DOI: 10.1016/j.actbio.2006.12.007

This study describes the effect of multilayered DNA coatings on (i) the formation of mineralized depositions from simulated body fluids (SBF); and (ii) osteoblast-like cell behavior with and without pretreatment in SBF. DNA coatings were generated using electrostatic self-assembly, with poly- d-lysine or poly(allylamine hydrochloride) as cationic polyelectrolytes, on titanium substrates. Coated substrates and non-coated controls were immersed in SBF with various compositions. The deposition of calcium phosphate was enhanced on multilayered DNA coatings as compared with non-coated controls, and was dependent on the type of cationic polyelectrolyte used in the build-up of the DNA coatings. Further analysis showed that the depositions consisted of carbonated apatite. Non-pretreated DNA coatings were found to have no effect on osteoblast-like cell behavior compared with titanium controls. On the other hand, SBF-pretreatment of DNA coatings affected the differentiation of osteoblast-like cells through an increased deposition of osteocalcin. The results of this study are indicative of the bone-bonding capacities of DNA coatings. Nevertheless, future animal experiments are required to provide conclusive evidence for the bioactivity of DNA coatings.